Organic Photodiodes for Biosensor Miniaturization

• Published in: Analytical chemistry (Washington) Vol. 81; no. 9; pp. 3455 - 3461
• Main Authors: Wojciechowski, Jason R, Shriver-Lake, Lisa C, Yamaguchi, Mariko Y, Füreder, Erwin, Pieler, Roland, Schamesberger, Martin, Winder, Christoph, Prall, Hans Jürgen, Sonnleitner, Max, Ligler, Frances S
• Format: Journal Article
• Language: English
• Published: Washington, DC American Chemical Society 01.05.2009
• Subjects: Analytical chemistry; Biological and medical sciences; Biosensing Techniques - instrumentation; Biosensing Techniques - methods; Biosensors; Biotechnology; Chemical and thermal methods; Chemistry; Dose-Response Relationship, Drug; Electrodes; Electronics; Enterotoxins - isolation & purification; Exact sciences and technology; Fundamental and applied biological sciences. Psychology; General, instrumentation; Glass - chemistry; Luminescence; Methods. Procedures. Technologies; Miniaturization; Miscellaneous; Organic Chemicals - chemistry; Pathogens; Polymers; Sensitivity and Specificity; Various methods and equipments; Disposable; Fluid mechanics; Antibody; Immobilization; Polymer; Chemiluminescence; Photodiode; Chemical sensor; Immunological method; Biosensor; Electronic component; Miniaturization; Microfluidics
• ISSN: 0003-2700; 1520-6882
• DOI: 10.1021/ac8027323

Biosensors have successfully demonstrated the capability to detect multiple pathogens simultaneously at very low levels. Miniaturization of biosensors is essential for use in the field or at the point of care. While microfluidic systems reduce the footprint for biochemical processing devices and electronic components are continually becoming smaller, optical components suitable for integration-such as LEDs and CMOS chips-are generally still too expensive for disposable components. This paper describes the integration of polymer diodes onto a biosensor chip to create a disposable device that includes both the detector and the sensing surface coated with immobilized capture antibody. We performed a chemiluminescence immunoassay on the OPD substrate and measured the results using a hand-held reader attached to a laptop computer. The miniaturized biosensor with the disposable slide including the organic photodiode detected Staphylococcal enterotoxin B at concentrations as low as 0.5 ng/mL.