Probing Elastic and Viscous Properties of Phospholipid Bilayers Using Neutron Spin Echo Spectroscopy

• Published in: The journal of physical chemistry letters Vol. 8; no. 19; pp. 4679 - 4684
• Main Authors: Nagao, Michihiro, Kelley, Elizabeth G, Ashkar, Rana, Bradbury, Robert, Butler, Paul D
• Format: Journal Article
• Language: English
• Published: United States American Chemical Society 05.10.2017
• Subjects: Cell Membrane - chemistry; Diffusion; Lipid Bilayers - chemistry; Molecular Dynamics Simulation; Neutrons; Phosphatidylcholines - chemistry; Phospholipids; Spectrum Analysis; Temperature
• ISSN: 1948-7185; 1948-7185
• DOI: 10.1021/acs.jpclett.7b01830

The elastic and viscous properties of self-assembled amphiphilic membranes dictate the intricate hierarchy of their structure and dynamics ranging from the diffusion of individual molecules to the large-scale deformation of the membrane. We previously demonstrated that neutron spin echo spectroscopy measurements of model amphiphilic membranes can access the naturally occurring submicrosecond membrane motions, such as bending and thickness fluctuations. Here we show how the experimentally measured fluctuation parameters can be used to determine the inherent membrane properties and demonstrate how membrane viscosity and compressibility modulus are influenced by lipid composition in a series of simple phosphatidylcholine bilayers with different tail lengths as a function of temperature. This approach highlights the interdependence of the bilayer elastic and viscous properties and the collective membrane dynamics and opens new avenues to investigating the mechanical properties of more complex and biologically inspired systems.