Grail Suppresses Stress‑induced Apoptosis and Colony Formation in Oral Cancer Cells

• Published in: Journal of Medical Sciences Vol. 39; no. 2; pp. 61 - 66
• Main Authors: Liu, Pei-Yao, Chen, Ying-Chuan
• Format: Journal Article
• Language: English
• Published: 台灣 國防醫學院 01.03.2019; Medknow Publications and Media Pvt. Ltd; Wolters Kluwer Medknow Publications
• Subjects: Analysis; Apoptosis; Biochemistry; Cancer; Cancer cells; Fluorescence; Gene expression; Genes; Genetic aspects; Grail; MEDLINE; Methylene blue; Mouth cancer; oral cancer cells; p53 and p21; Polymerase chain reaction; Scopus; Tumor proteins; Tumors; Grail; p53 and p21; oral cancer cells
• ISSN: 1011-4564
• DOI: 10.4103/jmedsci.jmedsci_53_18

Aims: The purpose of this study is to investigate the role of Grail in stress-induced apoptosis and tumorigenesis in oral cancer cells. Subjects and Methods: We analyzed gene expression of Grail in oral cancer cell lines (KB, SAS, SCC-4, and SCC-25) treated with 5-azadC or/and trichostatin A (TSA) by RT-PCR. The effects of Grail on the expression of p53 and p21 were analyzed by real-time polymerase chain reaction and Western blot for KB cells (KB/vector, KB/Grail and KB/shGrail). The anti-apoptotic effects of Grail were determined by fluorescence-activated cell sorting in KB/vector and KB/Grail cells. The effects of Grail on tumorigenesis were through clonogenic analysis in KB cells (KB/vector, KB/Grail and KB/shGrail). Results: Treatment with 5-azadC or/and TSA-induced Grail mRNA expression in oral cancer cells. Grail overexpression reduced p53 and p21 expression. Conversely, p53 and p21 expressions were increased in KB/shGrail cells. Furthermore, Grail can inhibit resveratrol-or etoposide-induced apoptosis in KB cells. Finally, Grail can significantly suppress colony formation in KB cells. Conclusions: The expression of Grail is epigenetically regulated in oral cancer cells. Grail can reduce p53 and p21 expression and stress-induced cell death and suppress the colony formation in KB cells.