Identification of frog photoreceptor plasma and disk membrane proteins by radioiodination

• Published in: Biochemistry (Easton) Vol. 26; no. 6; pp. 1769 - 1776
• Main Authors: Witt, Patricia L, Bownds, M. Deric
• Format: Journal Article
• Language: English
• Published: Washington, DC American Chemical Society 24.03.1987
• Subjects: Animals; Autoradiography; Biological and medical sciences; Cell Membrane - analysis; Cell physiology; Electrophoresis, Polyacrylamide Gel; Fundamental and applied biological sciences. Psychology; Iodine Radioisotopes; Membrane Proteins - analysis; Molecular and cellular biology; Molecular Weight; Photoreceptor Cells - analysis; Rana catesbeiana; Ranidae; Vision, photoreception; Iodination; Disk membrane; Vertebrata; Membrane protein; Outer segment; Frog; Vision; Plasma membrane; Amphibia; Retina; Salientia
• ISSN: 0006-2960; 1520-4995
• DOI: 10.1021/bi00380a040

Several functions have been identified for the plasma membrane of the rod outer segment, including control of light-dependent changes in sodium conductance and a sodium-calcium exchange mechanism. However, little is known about its constituent proteins. Intact rod outer segments substantially free of contaminants were prepared in the dark and purified on a density gradient of Percoll. Surface proteins were then labeled by lactoperoxidase-catalyzed radioiodination, and intact rod outer segments were reisolated. Membrane proteins were identified by polyacrylamide gel electrophoresis and autoradiography. The surface proteins labeled included rhodopsin, the major membrane protein, and 12 other proteins. Several control experiments indicated that the labeled proteins are integral membrane proteins and that label is limited to the plasma membrane. To compare the protein composition of plasma membrane with that of the internal disk membrane, purified rod outer segments were lysed by hypotonic disruption or freeze-thawing, and plasma plus disk membranes were radioiodinated. In these membrane preparations, rhodopsin was the major iodinated constituent, with 12 other proteins also labeled. Autoradiographic evidence indicated some differences in protein composition between disk and plasma membranes. A quantitative comparison of the two samples showed that labeling of two proteins, 24 kilodaltons (kDa) and 13 kDa, was enriched in the plasma membrane, while labeling of a 220-kDa protein was enriched in the disk membrane. These plasma membrane proteins may be associated with important functions such as the light-sensitive conductance and the sodium-calcium exchanger.