Surface-Induced Hydrogelation for Fluorescence and Naked-Eye Detections of Enzyme Activity in Blood

• Published in: Analytical chemistry (Washington) Vol. 88; no. 14; pp. 7318 - 7323
• Main Authors: Xu, Tengyan, Liang, Chunhui, Ji, Shenglu, Ding, Dan, Kong, Deling, Wang, Ling, Yang, Zhimou
• Format: Journal Article
• Language: English
• Published: United States American Chemical Society 19.07.2016
• Subjects: Alkaline Phosphatase - blood; Animals; Biological properties; Blood; Cell Line; Cells; Color; Enzyme activity; Enzymes; Enzymes - blood; Fluorescence; Fluorescent Dyes - chemistry; Glass; Humans; Hydrogels; Hydrogels - chemistry; Mice; Nitro Compounds - chemistry; Oxadiazoles - chemistry; Peptides - chemical synthesis; Peptides - chemistry; Self assembly; Spectrometry, Fluorescence; Surface chemistry
• ISSN: 0003-2700; 1520-6882
• DOI: 10.1021/acs.analchem.6b01660

Fluorescence probes have been widely applied for the detection of important analytes with high sensitivity and specificity. However, they cannot be directly applied for the detection in samples with autofluorescence such as blood. Herein, we demonstrated a simple but effective method of surface-induced self-assembly/hydrogelation for fluorescence detection of an enzyme in biological fluids including blood and cell lysates. The method utilizes an attracting glass surface to induce self-assembly of an enzyme-generating fluorescent probe. After removing the upper solution, the fluorescence turn-on at the glass surface can therefore be used for the detection of enzyme activity. By judging the thickness and color depth of hydrogels at the surface of the glass plates, we could also estimate the enzyme activity by naked eyes. Our study not only expands the application of molecular self-assembly but also provides a useful method that can be applied for direct detection of enzyme activity in complex biological samples such as blood and cell lysates.