Development of a Universal Colorimetric Indicator for G‑Quadruplex Structures by the Fusion of Thiazole Orange and Isaindigotone Skeleton

The rapid and convenient method for identification of all kinds of G-quadruplex is highly desirable. In the present study, a novel colorimetric indicator for a vast variety of G-quadruplex was designed and synthesized on the basis of thiazole orange and isaindigotone skeleton. Its distinct color cha...

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Published inAnalytical chemistry (Washington) Vol. 84; no. 15; pp. 6288 - 6292
Main Authors Yan, Jin-Wu, Ye, Wen-Jie, Chen, Shuo-Bin, Wu, Wei-Bin, Hou, Jin-Qiang, Ou, Tian-Miao, Tan, Jia-Heng, Li, Ding, Gu, Lian-Quan, Huang, Zhi-Shu
Format Journal Article
LanguageEnglish
Published United States American Chemical Society 07.08.2012
Subjects
Alkaloids - chemistry
Benzothiazoles - chemistry
Biosynthesis
Color
Colorimetry
Fluorescence Resonance Energy Transfer
G-Quadruplexes
Indicators and Reagents - chemistry
Measuring instruments
Molecular biology
Quinazolines - chemistry
Quinolines - chemistry
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Summary:The rapid and convenient method for identification of all kinds of G-quadruplex is highly desirable. In the present study, a novel colorimetric indicator for a vast variety of G-quadruplex was designed and synthesized on the basis of thiazole orange and isaindigotone skeleton. Its distinct color change enables label-free visual detection of G-quadruplexes, which is due to the disassembly of dye H-aggregates to monomers. This specific detection of G-quadruplex arises from its end-stacking interaction with G-quartet. On the basis of this universal indicator, a facile approach for large-scale identification of G-quadruplex was developed.
Bibliography:SourceType-Other Sources-1
ObjectType-Article-2
content type line 63
ObjectType-Correspondence-1
ISSN:0003-2700
1520-6882
DOI:10.1021/ac300207r