Detection and Separation of Gas-Phase Carbon-Centered Radicals from Cigarette Smoke and Diesel Exhaust

• Published in: Analytical chemistry (Washington) Vol. 70; no. 9; pp. 2008 - 2012
• Main Authors: Flicker, Thomas M, Green, Sarah A
• Format: Journal Article
• Language: English
• Published: Washington, DC American Chemical Society 01.05.1998
• Subjects: Analytical chemistry; Carbon; Chemical reactions; Chemistry; Chromatographic methods and physical methods associated with chromatography; Diesel fuels; Emissions; Exact sciences and technology; Gases; Other chromatographic methods; Smoking; Trace analysis; Radical trapping; Fluorescence detector; Chemical analysis; Multicomponent analysis; Nitroxyl; Organic free radical; HPLC chromatography; Exhaust gas; Derivatization
• ISSN: 0003-2700; 1520-6882
• DOI: 10.1021/ac970858f

Carbon-centered radicals were trapped from gas-phase cigarette smoke and diesel engine exhaust by reaction with a nitroxide, 3-amino-2,2,5,5-tetramethyl-1-pyrrolidinyloxy (3AP). The resulting mixture of stable, diamagnetic adducts was derivatized with naphthalenedicarboxaldehyde (NDA) to produce highly fluorescent products. Derivatives were separated by high-performance liquid chromatography (HPLC), which revealed distinctly different suites of radicals present in the two systems. Integration of HPLC peaks gave approximately 22 ± 7 nmol of radicals per cigarette and 3 ± 1 nmol of radicals per liter of diesel engine exhaust. An estimated 8−10 different carbon-centered radical species are present in each system.