Identification of Food-Derived Collagen Peptides in Human Blood after Oral Ingestion of Gelatin Hydrolysates

• Published in: Journal of agricultural and food chemistry Vol. 53; no. 16; pp. 6531 - 6536
• Main Authors: Iwai, Koji, Hasegawa, Takanori, Taguchi, Yasuki, Morimatsu, Fumiki, Sato, Kenji, Nakamura, Yasushi, Higashi, Akane, Kido, Yasuhiro, Nakabo, Yukihiro, Ohtsuki, Kozo
• Format: Journal Article
• Language: English
• Published: United States American Chemical Society 10.08.2005
• Subjects: Adult; Animals; blood chemistry; Chickens; Chromatography, High Pressure Liquid; collagen; Collagen - blood; Dietary Proteins - administration & dosage; food intake; gelatin; Gelatin - administration & dosage; Gelatin - pharmacokinetics; Humans; ingestion; intestinal absorption; metabolism; oligopeptides; peptides; Peptides - blood; protein hydrolysates; Protein Hydrolysates - administration & dosage; Protein Hydrolysates - pharmacokinetics; Swine
• ISSN: 0021-8561; 1520-5118
• DOI: 10.1021/jf050206p

In the present study, we identified several food-derived collagen peptides in human blood after oral ingestion of some gelatin hydrolysates. Healthy human volunteers ingested the gelatin hydrolysates (9.4−23 g) from porcine skin, chicken feet, and cartilage after 12 h of fasting. Negligible amounts of the peptide form of hydroxyproline (Hyp) were observed in human blood before the ingestion. After the oral ingestion, the peptide form of Hyp significantly increased and reached a maximum level (20−60 nmol/mL of plasma) after 1−2 h and then decreased to half of the maximum level at 4 h after the ingestion. Major constituents of food-derived collagen peptides in human serum and plasma were identified as Pro-Hyp. In addition, small but significant amounts of Ala-Hyp, Ala-Hyp-Gly, Pro-Hyp-Gly, Leu-Hyp, Ile-Hyp, and Phe-Hyp were contained. Keywords: Collagen; gelatin hydrolysates; Pro-Hyp; fibroblast; peptide; food; gelatin; skin; osteoporosis