Determination of Total N-Nitroso Compounds by Chemical Denitrosation Using CuCl

A method for the determination of total N-nitroso compounds (NOC) by chemical denitrosation and subsequent chemiluminescence detection of evolved NO is described. Denitrosation was accomplished with CuCl in HCl at 70 °C. The detection limit for N-nitrosoproline (NPRO) was 1 pmol. NO formation from N...

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Published inJournal of agricultural and food chemistry Vol. 53; no. 12; pp. 4686 - 4691
Main Authors Wang, Jia, Chan, W. Geoffrey, Haut, Stephen A, Krauss, Marc R, Izac, Richard R, Hempfling, Walter P
Format Journal Article
LanguageEnglish
Published Washington, DC American Chemical Society 15.06.2005
Subjects
Biological and medical sciences
carcinogens
chemical analysis
chemical reactions
chlorides
copper
Copper - chemistry
copper chloride
denitrosation
food contamination
Food industries
foods
Fundamental and applied biological sciences. Psychology
Hydrochloric Acid
Luminescent Measurements
Nitric Oxide - analysis
Nitric Oxide - chemistry
nitroso compounds
Nitroso Compounds - analysis
Nitroso compound
NO
Nitric oxide
Chemical compound
CuCI
Chemiluminescence
Radical Purger
Total N-nitroso compounds
Analyzer
chemical denitrosation
Nitric Oxide Analyzer (NOA 280)
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Summary:A method for the determination of total N-nitroso compounds (NOC) by chemical denitrosation and subsequent chemiluminescence detection of evolved NO is described. Denitrosation was accomplished with CuCl in HCl at 70 °C. The detection limit for N-nitrosoproline (NPRO) was 1 pmol. NO formation from NPRO was linear (R 2 = 0.999) from 4 pmol to 2 nmol. Among the possible interfering compounds tested, only S-nitroso compounds contribute any significant interference. This method had several advantages over other similar methods:  (1) A commercially available one-piece reaction vessel and a NO analyzer with software were used. (2) NO release occurred rapidly and was easily measured and quantified. (3) Compared to HBr or HI, CuCl was more convenient to work with and safe. (4) CuCl was suitable for samples in aqueous and most organic solvents. The application of this method to food, personal care products, and human body fluids demonstrates its utility. Keywords: Total N-nitroso compounds; chemical denitrosation; chemiluminescence; CuCl; NO; Nitric Oxide Analyzer (NOA 280); Radical Purger
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ISSN:0021-8561
1520-5118
DOI:10.1021/jf0481709