Inteins as Traceless Purification Tags for Unnatural Amino Acid Proteins

The expression of proteins containing unnatural amino acids through suppression of a stop codon can be limited by truncation due to competition with release factors. When the site of incorporation is near the C-terminus, it may not be feasible to separate the full-length unnatural amino acid protein...

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Bibliographic Details
Published inJournal of the American Chemical Society Vol. 137; no. 5; pp. 1734 - 1737
Main Authors Batjargal, Solongo, Walters, Christopher R, Petersson, E. James
Format Journal Article
LanguageEnglish
Published United States American Chemical Society 11.02.2015
Subjects
Amino Acids
Archaeal Proteins - chemistry
Archaeal Proteins - isolation & purification
Inteins
Models, Molecular
Protein Conformation
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Summary:The expression of proteins containing unnatural amino acids through suppression of a stop codon can be limited by truncation due to competition with release factors. When the site of incorporation is near the C-terminus, it may not be feasible to separate the full-length unnatural amino acid protein from the truncated form. We report a simple, traceless procedure that allows one to isolate the desired protein using a C-terminal intein fusion.
Bibliography:ObjectType-Article-1
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ISSN:0002-7863
1520-5126
DOI:10.1021/ja5103019