Antioxidant Activity of Phenolics in Leaves of Three Red Pepper (Capsicum annuum) Cultivars

• Published in: Journal of agricultural and food chemistry Vol. 62; no. 4; pp. 850 - 859
• Main Authors: Kim, Woo-Ri, Kim, Eun Ok, Kang, Kyungsu, Oidovsambuu, Sarangerel, Jung, Sang Hoon, Kim, Byung Sup, Nho, Chu Won, Um, Byung-Hun
• Format: Journal Article
• Language: English
• Published: United States American Chemical Society 29.01.2014; American Chemical Society, Books and Journals Division
• Subjects: 2,2-diphenyl-1-picrylhydrazyl; anthocyanins; Anthocyanins - isolation & purification; Anthocyanins - pharmacology; antioxidant activity; Antioxidants - analysis; Antioxidants - isolation & purification; Antioxidants - pharmacology; apigenin; Capsicum - chemistry; Capsicum annuum; chlorogenic acid; cultivars; ethanol; free radical scavengers; Hep G2 Cells; human cell lines; Humans; leaves; oxidative stress; Oxidative Stress - drug effects; pepper; Phenols - analysis; Phenols - pharmacology; Plant Extracts - chemistry; Plant Extracts - pharmacology; Plant Leaves - chemistry; polyamines; Species Specificity; tert-Butylhydroperoxide - pharmacology; phenolic compounds; Capsicum annuum; anthocyanin; red pepper leaves; antioxidant effects
• ISSN: 0021-8561; 1520-5118
• DOI: 10.1021/jf403006c

The antioxidant properties and phenolic profiles were first investigated in this paper on the leaves of three red pepper cultivars, Blackcuban (BCPL), Hongjinju (HPL), and Yeokgang-hongjanggun (YHPL). Of the ethanol extract of the three cultivars, BCPL showed potent antioxidant activities against the 1,1-diphenyl-2-picrylhydrazyl radical (DPPH) and the 2,2-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) radical. Nine antioxidative compounds from the red pepper leaves were isolated and identified as one polyamine phenolic conjugate, N-caffeoylputrescine (1); three chlorogenic acid derivatives, 5-O-caffeoylquinic acid (2), 5-O-caffeoylquinic acid methyl ester (4), and 5-O-caffeoylquinic acid butyl ester (9); one anthocyanin, delphinidin-3-[4-trans-coumaroyl-l-rhamnosyl(1→6)glucopyranoside]-5-O-glucopyranoside (3); and four flavone glycosides, luteolin-7-O-apiofuranosyl(1→2)glucopyranoside (5), luteolin-7-O-glucopyranoside (6), apigenin 7-O-apiofuranosyl(1→2)glucopyranoside (7), apigenin-7-O-glucopyranoside (8). 1 and 3 had the greatest potential for radical-scavenging activity and HepG2 cells protecting effect against oxidative stress. BCPL exhibited the highest content of 1 and 3. Of the three cultivars BCPL may be considered a good source of antioxidants.