Modulation of Adenosine Receptors by [60]Fullerene Hydrosoluble Derivative in SK-N-MC Cells

• Published in: ACS chemical neuroscience Vol. 2; no. 7; pp. 363 - 369
• Main Authors: Giust, Davide, León, David, Ballesteros-Yañez, Inmaculada, Da Ros, Tatiana, Albasanz, José Luis, Martín, Mairena
• Format: Journal Article
• Language: English
• Published: United States American Chemical Society 20.07.2011
• Subjects: Adenosine A2 Receptor Agonists - chemical synthesis; Adenosine A2 Receptor Agonists - pharmacology; Adenosine A2 Receptor Antagonists - chemical synthesis; Adenosine A2 Receptor Antagonists - pharmacology; Blotting, Western; Cell Line, Tumor; Cell Membrane - metabolism; Cell Survival - drug effects; Coloring Agents; DNA, Complementary - biosynthesis; DNA, Complementary - genetics; Fullerenes - chemistry; Fullerenes - pharmacology; Humans; Isomerism; Microscopy, Electron; Real-Time Polymerase Chain Reaction; Receptor, Adenosine A1 - drug effects; Receptor, Adenosine A2A - drug effects; Receptors, Purinergic P1 - drug effects; RNA - genetics; RNA - isolation & purification; Solubility; Tetrazolium Salts; Thiazoles; SK-N-MC cells; fullerene; Adenosine receptor
• ISSN: 1948-7193; 1948-7193
• DOI: 10.1021/cn200016q

The most known fullerenes are spherical carbon compounds composed of 60 carbon atoms. C60 fullerenes have shown biochemical and biomedical properties in the last years such as as blockade of apoptosis and neuroprotection. The nucleoside adenosine has a neuroprotective role mainly due to inhibition of glutamate release, which is a neurotransmitter related to excitotoxicity and cell death. In the present work, we have determined the presence of adenosine receptors in SK-N-MC cells, a neuroepithelioma human cell line, and analyzed the effect of fullerenes in these receptors by using radioligand binding, immunoblotting, and quantitative real time PCR assays. Results demonstrated that SK-N-MC cells endogenously express adenosine receptors. Fullerene exposure of these cells did not affect cell viability measured by MTT reduction assay. However, adenosine A1 and A2A receptors were both increased in SK-N-MC cells after treatment. These results suggest for the first time the modulation of adenosine receptors after C60 fullerenes exposure.