Actin Architecture Steers Microtubules in Active Cytoskeletal Composite

• Published in: Nano letters Vol. 22; no. 21; pp. 8584 - 8591
• Main Authors: Kučera, Ondřej, Gaillard, Jérémie, Guérin, Christophe, Utzschneider, Clothilde, Théry, Manuel, Blanchoin, Laurent
• Format: Journal Article
• Language: English
• Published: American Chemical Society 09.11.2022
• Subjects: Actin Cytoskeleton; Actins; Cellular Biology; Cytoskeleton; Life Sciences; Microtubules; Nanotechnology; gliding assay; actin filaments; cytoskeletal composite; microtubules; network architecture
• ISSN: 1530-6984; 1530-6992
• DOI: 10.1021/acs.nanolett.2c03117

Motility assays use surface-immobilized molecular motors to propel cytoskeletal filaments. They have been widely used to characterize motor properties and their impact on cytoskeletal self-organization. Moreover, the motility assays are a promising class of bioinspired active tools for nanotechnological applications. While these assays involve controlling the filament direction and speed, either as a sensory readout or a functional feature, designing a subtle control embedded in the assay is an ongoing challenge. Here, we investigate the interaction between gliding microtubules and networks of actin filaments. We demonstrate that the microtubule’s behavior depends on the actin architecture. Both unbranched and branched actin decelerate microtubule gliding; however, an unbranched actin network provides additional guidance and effectively steers the microtubules. This effect, which resembles the recognition of cortical actin by microtubules, is a conceptually new means of controlling the filament gliding with potential application in the design of active materials and cytoskeletal nanodevices.