Quantification of Jasmonic Acid by SPME in Tomato Plants Stressed by Ozone

• Published in: Journal of agricultural and food chemistry Vol. 54; no. 25; pp. 9317 - 9321
• Main Authors: Zadra, Claudia, Borgogni, Andrea, Marucchini, Cesare
• Format: Journal Article
• Language: English
• Published: Washington, DC American Chemical Society 13.12.2006
• Subjects: Biological and medical sciences; Chromatography, Gas; Cyclopentanes - analysis; Food industries; Fruit and vegetable industries; Fumigation; Fundamental and applied biological sciences. Psychology; Gas Chromatography-Mass Spectrometry; headspace analysis; headspace solid phase microextraction; jasmonic acid; leaves; Lycopersicon esculentum - chemistry; Lycopersicon esculentum - drug effects; methyl jasmonate; microextraction; Oxylipins; ozone; Ozone - pharmacology; phytotoxicity; Plant Growth Regulators - analysis; Plant Leaves - chemistry; plant stress; Sensitivity and Specificity; Solanum lycopersicum var. lycopersicum; solid phase extraction; tomatoes; Vegetables; HS-SPME; Dicotyledones; Angiospermae; Lycopersicon esculentum; Ozone; Spermatophyta; Solanaceae; Tomato; Stress; Jasmonic acid; Quantitative analysis
• ISSN: 0021-8561; 1520-5118
• DOI: 10.1021/jf062249r

Jasmonates are signalling molecules induced in plants as a response to various biotic and/or abiotic stresses. As ozone is known to activate defense responses in plants, we have monitored the concentration of jasmonic acid in tomato leaves during and after an acute exposure to this abiotic elicitor. In this experiment, we observed that the maximum induction of jasmonic acid in O3-fumigated plants occurred 9 h after the end of treatment and the concentration of jasmonic acid in stressed plants increased 13-fold. However, the level of endogenous methyl-jasmonate was constant during the observed period. The extraction and quantification of jasmonic acid as its methyl ester was performed by headspace-solid-phase microextraction (or HS-SPME) in combination with GC−FID and GC−MS. The sensitivity (LOD = 2 ng/g) of this method permitted the detection and quantification of jasmonic acid present in plant tissues at very low concentrations. Keywords: Tomato; jasmonic acid; ozone; HS-SPME