Introducing Biobased Ionic Liquids as the Nonaqueous Media for Enzymatic Synthesis of Phosphatidylserine

• Published in: Journal of agricultural and food chemistry Vol. 63; no. 5; pp. 1558 - 1561
• Main Authors: Bi, Yan-Hong, Duan, Zhang-Qun, Li, Xiang-Qian, Wang, Zhao-Yu, Zhao, Xi-Rong
• Format: Journal Article
• Language: English
• Published: United States American Chemical Society 11.02.2015; American Chemical Society, Books and Journals Division
• Subjects: anions; Bacterial Proteins - chemistry; Biocatalysis; biocompatible materials; enzyme activity; ionic liquids; Ionic Liquids - chemistry; neutralization; phosphatidylcholines; Phosphatidylcholines - chemistry; phosphatidylserines; Phosphatidylserines - chemical synthesis; Phospholipase D - chemistry; serine; Serine - chemistry; Streptomyces - enzymology; phosphatidylserine; phospholipase D; biobased ionic liquids; transphosphatidylation
• ISSN: 0021-8561; 1520-5118
• DOI: 10.1021/jf505296k

Biobased ionic liquids with cholinium as the cation and amino acids as the anions, which could be prepared from renewable biomaterials by simple neutralization reactions, have recently been described as promising and green solvents. Herein, they were successfully used as the reaction media for enzyme-mediated transphosphatidylation of phosphatidylcholine with l-serine for phosphatidylserine synthesis for the first time. Our results indicated that the highest phosphatidylserine yield of 86.5% was achieved. Moreover, 75% original activity of the enzyme was maintained after being used for 10 batches. The present work could be considered an alternative enzymatic strategy for preparing phosphatidylserine. Additionally, the excellent results make the biobased ionic liquids more promising candidates for use as environmentally friendly solvents in biocatalysis fields.