Antifouling Peptide Hydrogel Based Electrochemical Biosensors for Highly Sensitive Detection of Cancer Biomarker HER2 in Human Serum

A facile strategy for the electrochemical detection of human epidermal growth factor receptor 2 (HER2), a breast cancer biomarker, was presented via the fabrication of an antifouling sensing interface based on the conducting polymer poly­(3,4-ethylenedioxythiophene) (PEDOT) and a biocompatible pepti...

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Published inAnalytical chemistry (Washington) Vol. 93; no. 19; pp. 7355 - 7361
Main Authors Wang, Wenqi, Han, Rui, Chen, Min, Luo, Xiliang
Format Journal Article
LanguageEnglish
Published United States American Chemical Society 18.05.2021
Subjects
adsorption
Analytical chemistry
Antifouling
Antifouling substances
Biocompatibility
Biofouling
Biological properties
Biological samples
Biomarkers
Biomolecules
Biosensors
blood serum
Breast cancer
breast neoplasms
Chemistry
Conducting polymers
detection limit
Electrochemical analysis
Electrochemistry
Epidermal growth factor
ErbB-2 protein
erbB-2 receptor
Fabrication
Fluorene
fluorenes
Growth factors
humans
Hydrogels
hydrophilicity
Peptides
Polymers
Substrates
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Summary:A facile strategy for the electrochemical detection of human epidermal growth factor receptor 2 (HER2), a breast cancer biomarker, was presented via the fabrication of an antifouling sensing interface based on the conducting polymer poly­(3,4-ethylenedioxythiophene) (PEDOT) and a biocompatible peptide hydrogel. The peptide hydrogel prepared from a designed short peptide of Phe-Glu-Lys-Phe functionalized with a fluorene methoxycarbonyl group (Fmoc-FEKF) enabled excellent activity preservation for the immobilized biomolecules, and its good hydrophilicity facilitated effective alleviation of nonspecific adsorption or biofouling, while the PEDOT film provided a highly stable and conducting substrate. The developed biosensor was highly sensitive and selective for HER2 detection, with a wide linear response range from 0.1 ng mL–1 to 1.0 μg mL–1 and a low limit of detection of 45 pg mL–1. Moreover, the peptide hydrogel based biosensor was feasible to use for complex biological samples, and it was capable of detecting HER2 in human serum with clinically acceptable accuracy, manifesting a promising potential for practical application.
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ISSN:0003-2700
1520-6882
1520-6882
DOI:10.1021/acs.analchem.1c01350