Antioxidant Activities of Abietane Diterpenoids Isolated from Torreya nucifera Leaves

• Published in: Journal of agricultural and food chemistry Vol. 54; no. 15; pp. 5369 - 5374
• Main Authors: Lee, Woo Song, Kim, Ju-Ryoung, Han, Jong-Min, Jang, Ki Chang, Sok, Dai-Eun, Jeong, Tae-Sook
• Format: Journal Article
• Language: English
• Published: WASHINGTON American Chemical Society 26.07.2006; Amer Chemical Soc
• Subjects: abietane diterpenoids; Agriculture; Agriculture, Multidisciplinary; antioxidant activity; antioxidants; Antioxidants - chemistry; Antioxidants - pharmacology; atherosclerosis; Biological and medical sciences; cell lines; chemical structure; Chemistry; Chemistry, Applied; Copper - pharmacology; Diterpenes, Abietane - chemistry; Diterpenes, Abietane - isolation & purification; Diterpenes, Abietane - pharmacology; diterpenoids; enzyme activity; Food industries; Food Science & Technology; free radical scavengers; free radicals; Fundamental and applied biological sciences. Psychology; leaves; Life Sciences & Biomedicine; Lipid Peroxidation - drug effects; Lipoproteins, LDL - chemistry; low density lipoprotein; macrophages; mice; nitric oxide; nitric oxide synthase; oxidation; Physical Sciences; Plant Leaves - chemistry; Science & Technology; Taxaceae - chemistry; Torreya nucifera; atherosclerosis; OXIDATION; NO; CHOLESTEROL; Torreya nucifera; LOW-DENSITY-LIPOPROTEIN; antioxidant activity; ROSMARINUS-OFFICINALIS L; abietane diterpenoids; LDL; NITRIC-OXIDE; iNOS; DEGRADATION; SMOOTH-MUSCLE-CELLS; CONSTITUENTS; Terpenoid; Cardiovascular disease; Plant leaf; Antioxidant; Vascular disease; Plant part; Atherosclerosis
• ISSN: 0021-8561; 1520-5118
• DOI: 10.1021/jf060896c

Investigation on antioxidant compounds from the ethanolic extracts of Torreya nucifera leaves resulted in the isolation of abietane diterpenoids, a known 18-methylesterferruginol (1) and a new 18-dimethoxyferruginol (2). The structures of compounds 1 and 2 were elucidated on the basis of their spectroscopic analyses. Compounds 1 and 2 inhibited the Cu2+-mediated, 2,2‘-azobis(2-amidinopropane)hydrochloride-mediated and 3-morpholinosydnonimine-1-mediated low-density lipoprotein (LDL) oxidation in the thiobarbituric acid-reactive substances assay as well as the macrophage-mediated LDL oxidation. Compounds 1 and 2 exhibited the potent antioxidant activities in the conjugated diene production, relative electrophoretic mobility, and apoB-100 fragmentation on copper-mediated LDL oxidation. Compound 1 also suppressed nitric oxide production and inducible nitric oxide synthase expression in lipopolysaccharide-stimulated RAW264.7 cells. Keywords: Torreya nucifera; abietane diterpenoids; LDL; antioxidant activity; NO; iNOS; atherosclerosis