Flow-Mediated Stem Cell Labeling with Superparamagnetic Iron Oxide Nanoparticle Clusters

This study presents a strategy to enhance the uptake of superparamagnetic iron oxide nanoparticle (SPIO) clusters by manipulating the cellular mechanical environment. Specifically, stem cells exposed to an orbital flow ingested almost a 2-fold greater amount of SPIO clusters than those cultured stat...

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Bibliographic Details
Published inACS applied materials & interfaces Vol. 5; no. 20; pp. 10266 - 10273
Main Authors Clay, Nicholas, Baek, Kwanghyun, Shkumatov, Artem, Lai, Mei-Hsiu, Smith, Cartney E, Rich, Max, Kong, Hyunjoon
Format Journal Article
LanguageEnglish
Published United States American Chemical Society 23.10.2013
Subjects
Animals
Aspartic Acid - analogs & derivatives
Aspartic Acid - chemical synthesis
Aspartic Acid - chemistry
Bone Marrow Cells - cytology
Cell Tracking
Cells, Cultured
Contrast Media - chemistry
Endocytosis
Ferric Compounds - chemistry
Magnetic Resonance Imaging
Magnetite Nanoparticles - chemistry
Mechanotransduction, Cellular
Mesenchymal Stem Cells - chemistry
Mesenchymal Stem Cells - cytology
Mice
Peptides - chemical synthesis
Peptides - chemistry
receptor-mediated endocytosis
MR imaging
iron oxide nanoparticles
cell tracking
mechanotransduction
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Summary:This study presents a strategy to enhance the uptake of superparamagnetic iron oxide nanoparticle (SPIO) clusters by manipulating the cellular mechanical environment. Specifically, stem cells exposed to an orbital flow ingested almost a 2-fold greater amount of SPIO clusters than those cultured statically. Improvements in magnetic resonance (MR) contrast were subsequently achieved for labeled cells in collagen gels and a mouse model. Overall, this strategy will serve to improve the efficiency of cell tracking and therapies.
ISSN:1944-8244
1944-8252
DOI:10.1021/am4030998