Quantification of Alkylresorcinol Metabolites in Plasma by High-Performance Liquid Chromatography with Coulometric Electrode Array Detection

• Published in: Journal of agricultural and food chemistry Vol. 56; no. 17; pp. 7678 - 7681
• Main Authors: Koskela, Anja, Samaletdin, Adile, Aubertin-Leheudre, Mylène, Adlercreutz, Herman
• Format: Journal Article
• Language: English
• Published: Washington, DC American Chemical Society 10.09.2008
• Subjects: Adult; Alkylation; alkylresorcinols; Analytical Methods; Biological and medical sciences; biomarkers; blood chemistry; blood plasma; Chromatography, High Pressure Liquid - methods; coulometric electrode array detection; Electrodes; epidemiological studies; Female; Food industries; Fundamental and applied biological sciences. Psychology; General aspects; high performance liquid chromatography; Humans; Hydroxybenzoates - blood; Hydroxybenzoates - urine; metabolites; Methods of analysis, processing and quality control, regulation, standards; Middle Aged; nutrient intake; Phenols; Phenylpropionates; Propionates - blood; Propionates - urine; quantitative analysis; Reproducibility of Results; Resorcinols - metabolism; Sensitivity and Specificity; whole grain foods; Electrodes; Analysis method; Metabolite; HPLC chromatography; Control method; Detection; Quantitative analysis
• ISSN: 0021-8561; 1520-5118
• DOI: 10.1021/jf801252s

This study presents the optimization and validation of a rapid protocol for quantifying alkyresorcinol (AR) metabolites 3,5-dihydroxybenzoic acid (DHBA) and 3-(3,5-dihydroxyphenyl)-1-propanoic acid (DHPPA) in plasma, using high-performance liquid chromatography (HPLC) coupled with a coulometric electrode array detector. Syringic acid (SyrA) serves as the internal standard. The new method is simple and could be used in large epidemiological studies. The summed AR metabolite concentrations measured in plasma correlate significantly with the summed urinary AR metabolite concentrations (R = 0.613; p < 0.001) and with the summed intact AR (C17:0−C25:0) concentrations in plasma (R = 0.686; p < 0.001). Additional investigation is needed to clarify whether the two plasma AR metabolites are useful as biomarkers of whole-grain intake and helpful in the exploration of the association between whole-grain cereal intake and human diseases.