Common links in the structure and cellular localization of Rhizobium chitolipooligosaccharides and general Rhizobium membrane phospholipid and glycolipid components

• Published in: Biochemistry (Easton) Vol. 34; no. 13; pp. 4467 - 4477
• Main Authors: Cedergren, Robert A, Lee, Jeongrim, Ross, Kerry L, Hollingsworth, Rawle I
• Format: Journal Article
• Language: English
• Published: United States American Chemical Society 04.04.1995
• Subjects: Carbohydrate Conformation; Chromatography, Gas; Chromatography, Gel; ESTRUCTURA QUIMICA; Gas Chromatography-Mass Spectrometry; Glycolipids - chemistry; LIPOPOLISACARIDOS; LIPOPOLYSACCHARIDE; Lipopolysaccharides - chemistry; Magnetic Resonance Spectroscopy; Membrane Lipids - chemistry; Methylation; Phospholipids - chemistry; RHIZOBIUM; Rhizobium meliloti; Sinorhizobium meliloti - chemistry; STRUCTURE CHIMIQUE; Sulfates - chemistry; Sulfates - metabolism
• ISSN: 0006-2960; 1520-4995
• DOI: 10.1021/bi00013a040

Several common links between the structural chemistry of the chitolipooligosaccharides of Rhizobium and the general rhizobial membrane lipid and lipopolysaccharide chemistry of these bacteria have been uncovered. Aspects of common chemistry include sulfation, methylation, and the position and extent of fatty acyl chain unsaturation. We find that bacteria which are known to synthesize sulfated chitolipooligosaccharides (such as Rhizobium meliloti strains and the broad-host-range Rhizobium species strain NGR234) also have sulfated lipopolysaccharides. Their common origins of sulfation have been demonstrated by using mutants which are known to be impaired in sulfating their chitolipooligosaccharides. In such cases, there is a corresponding diminution or complete lack of sulfation of the lipopolysaccharides. The structural diversity of the fatty acids observed in the chitolipooligosaccharides is also observed in the other membrane lipids. For instance, the doubly unsaturated fatty acids which are known to be predominant components of R. meliloti chitolipooligosaccharides were also found in the usual phospholipids and glycolipids. Also, the known functionalization of the chitolipooligosaccharides of R. sp. NGR234 by O-and N-methylation was also reflected in the lipopolyeaccharide of this organism. The common structural features of chitolipooligosaccharides and membrane components are consistent with a substantial degree of biosynthetic overlap and a large degree of cellular, spatial overlap between these molecules. The latter aspect is clearly demonstrated here since we show that the chitolipooligosaccharides are, in fact, normal membrane components of Rhizobium. This increases the importance of understanding the role of the bacterial cell surface chemistry in the Rhizobium/legume symbiosis and developing a comprehensive understanding of the highly integrated membrane lipid and glycolipid chemistry of Rhizobium