β‑Cyclodextrin-Functionalized Chitosan/Alginate Compact Polyelectrolyte Complexes (CoPECs) as Functional Biomaterials with Anti-Inflammatory Properties

• Published in: ACS applied materials & interfaces Vol. 10; no. 35; pp. 29347 - 29356
• Main Authors: Hardy, Alexandre, Seguin, Cendrine, Brion, Anaïs, Lavalle, Philippe, Schaaf, Pierre, Fournel, Sylvie, Bourel-Bonnet, Line, Frisch, Benoît, De Giorgi, Marcella
• Format: Journal Article
• Language: English
• Published: United States American Chemical Society 05.09.2018; Washington, D.C. : American Chemical Society
• Subjects: Alginic Acid - chemistry; Alginic Acid - pharmacology; Animals; Anti-Inflammatory Agents - chemistry; Anti-Inflammatory Agents - pharmacology; beta-Cyclodextrins - chemistry; beta-Cyclodextrins - pharmacology; Biocompatible Materials - chemistry; Biocompatible Materials - pharmacology; Bioengineering; Biomaterials; Chemical Sciences; Chitosan - chemistry; Chitosan - pharmacology; Galenic pharmacology; Life Sciences; Macrophages - drug effects; Mice; Pharmaceutical sciences; Polyelectrolytes - chemistry; Polyelectrolytes - pharmacology; Polymers; alginate; drug carriers; chitosan; natural biomaterials; CoPECs; anti-inflammatory; cyclodextrins; antiinflammatory
• ISSN: 1944-8244; 1944-8252
• DOI: 10.1021/acsami.8b09733

Nowadays, the need for therapeutic biomaterials displaying anti-inflammatory properties to fight against inflammation-related diseases is continuously increasing. Compact polyelectrolyte complexes (CoPECs) represent a new class of materials obtained by ultracentrifugation of a polyanion/polycation complex suspension in the presence of salt. Here, a noncytotoxic β-cyclodextrin-functionalized chitosan/alginate CoPEC was formulated, characterized, and described as a promising drug carrier displaying an intrinsic anti-inflammatory property. This new material was successfully formed, and due to the presence of cyclodextrins, it was able to trap and release hydrophobic drugs such as piroxicam used as a model drug. The intrinsic anti-inflammatory activity of this CoPEC was analyzed in vitro using murine macrophages in the presence of lipopolysaccharide (LPS) endotoxin. In this model, it was shown that CoPEC inhibited LPS-induced TNF-α and NO release and moderated the differentiation of LPS-activated macrophages. Over time, this kind of bioactive biomaterial could constitute a new family of delivery systems and expand the list of therapeutic tools available to target inflammatory chronic diseases such as arthritis or Crohn’s disease.