Transcriptomic profiling of haloarchaeal denitrification through RNA-Seq analysis

• Published in: Applied and environmental microbiology Vol. 90; no. 6; p. e0057124
• Main Authors: Miralles-Robledillo, Jose María, Martínez-Espinosa, Rosa María, Pire, Carmen
• Format: Journal Article
• Language: English
• Published: United States American Society for Microbiology 18.06.2024
• Subjects: Archaeal Proteins - genetics; Archaeal Proteins - metabolism; Denitrification; Denitrification - genetics; Detoxification; Gene Expression Profiling; Gene Expression Regulation, Archaeal; Gene sequencing; Genes; Genetics and Molecular Biology; Genomics and Proteomics; Haloferax mediterranei - genetics; Haloferax mediterranei - metabolism; Homeostasis; Molecular biology; Molecular modelling; Proteins; Ribonucleic acid; RNA; RNA-Seq; Transcription factors; Transcriptome; Transcriptomes; Transcriptomics; transcriptomics; denitrification; haloarchaea; transcriptional regulation; Haloferax mediterranei
• ISSN: 0099-2240; 1098-5336; 1098-5336
• DOI: 10.1128/aem.00571-24

Denitrification, a fundamental process within the nitrogen cycle, has been subject to extensive investigation due to its close association with anthropogenic activities, and its contribution to the global warming issue, mainly through the release of N 2 O emissions. Although our comprehension of denitrification and its implications is generally well established, most studies have been conducted in non-extreme environments with mesophilic microorganisms. Consequently, there is a significant knowledge gap concerning extremophilic denitrifiers, particularly those inhabiting hypersaline environments. The significance of this research was to delve into the process of haloarchaeal denitrification, utilizing the complete denitrifier haloarchaeon Haloferax mediterranei as a model organism. This research led to the analysis of the metabolic state of this microorganism under denitrifying conditions and the identification of regulatory signals and genes encoding proteins potentially involved in this pathway, serving as a valuable resource for future molecular studies.