Determination of Inorganic Fiber Density in Human Lung Tissue by Scanning Electron Microscopy after Low Temperature Ashing

The concentration of asbestos fibers in lung tissue may give information about previous occupational asbestos exposure. A procedure for the preparation, quantification and identification of asbestos fibers in human tissue is presented in this report. The procedure is based on low-temperature plasma...

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Bibliographic Details
Published inScandinavian Journal of Work, Environment ＆ Health Vol. 5; no. 2; pp. 151 - 157
Main Authors Gylseth, B, Ophus, E M, Mowé, G
Format Journal Article
LanguageEnglish
Published Finland Scandinavian Journal of Work, Environment ＆ Health 01.06.1979
National Board of Occupational Safety and Health
The Working Environment Fund
Swedish Medical Society, Section for Environmental Health
Work Research Institutes
Institute of Occupational Health
Subjects
Asbestos
Asbestos - analysis
Environmental Exposure
Humans
Insulation
Low temperature
Lung - analysis
Lungs
Magnification
Materials
Mesothelioma
Microscopy, Electron, Scanning
Occupational Medicine
P branes
Scanning electron microscopy
Temperature
Tissue samples
scanning electron microscopy
asbestos
lung tissue
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Summary:The concentration of asbestos fibers in lung tissue may give information about previous occupational asbestos exposure. A procedure for the preparation, quantification and identification of asbestos fibers in human tissue is presented in this report. The procedure is based on low-temperature plasma ashing of dry tissue, examination of the ash by scanning electron microscopy, and energy dispersive X-ray microanalysis. The advantages and disadvantages of this method are also discussed briefly.
ISSN:0355-3140
1795-990X
DOI:10.5271/sjweh.2657