Preparation of Translationally Competent tRNA by Direct Chemical Acylation

Nonsense codon suppression for unnatural amino acid incorporation requires the preparation of a suppressor aminoacyl-tRNA. Chemical acylation strategies are general but inefficient and arduous. A recent report (J. Am. Chem. Soc. 2007, 129, 15848) showed acylation of RNA mediated by lanthanum(III) us...

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Published inOrganic letters Vol. 12; no. 17; pp. 3776 - 3779
Main Authors Duffy, Noah H, Dougherty, Dennis A
Format Journal Article
LanguageEnglish
Published WASHINGTON American Chemical Society 03.09.2010
Amer Chemical Soc
Subjects
Acylation
Animals
Chemistry
Chemistry, Organic
Codon, Nonsense - genetics
Lanthanum - chemistry
Mice
Molecular Structure
Oocytes - metabolism
Physical Sciences
RNA, Transfer - chemical synthesis
RNA, Transfer - chemistry
RNA, Transfer, Amino Acyl - chemical synthesis
RNA, Transfer, Amino Acyl - chemistry
Science & Technology
Xenopus - genetics
Xenopus - metabolism
IMPROVED AMBER
SYSTEM
SUPPRESSOR TRANSFER-RNAS
IN-VIVO INCORPORATION
DYNAMICS
UNNATURAL AMINO-ACIDS
AMINOACYLATION
BINDING
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Summary:Nonsense codon suppression for unnatural amino acid incorporation requires the preparation of a suppressor aminoacyl-tRNA. Chemical acylation strategies are general but inefficient and arduous. A recent report (J. Am. Chem. Soc. 2007, 129, 15848) showed acylation of RNA mediated by lanthanum(III) using amino acid phosphate esters. The successful implementation of this methodology to full-length suppressor tRNA is described, and it is shown that the derived aminoacyl-tRNA is translationally competent in Xenopus oocytes.
Bibliography:NIH RePORTER
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ISSN:1523-7060
1523-7052
DOI:10.1021/ol101408f