Transient Isotachophoretic−Electrophoretic Separations of Lanthanides with Indirect Laser-Induced Fluorescence Detection

• Published in: Analytical chemistry (Washington) Vol. 70; no. 13; pp. 2475 - 2480
• Main Authors: Church, Matthew N, Spear, Jonathan D, Russo, Richard E, Klunder, Gregory L, Grant, Patrick M, Andresen, Brian D
• Format: Journal Article
• Language: English
• Published: Washington, DC American Chemical Society 01.07.1998
• Subjects: Analytical chemistry; Chemistry; Chromatographic methods and physical methods associated with chromatography; Exact sciences and technology; Fluorescence; Lasers; Other chromatographic methods; Trace analysis; Capillary column; Fluorescence detector; Chemical analysis; Zone electrophoresis; Electrolyte solution; Sulfato complex; Organic ligand; Isotachophoresis; Chemical enrichment; Multicomponent analysis; Samarium Complexes; Concentration effect; Preconcentration; Aqueous solution; Lanthanide Complexes; Cerium Complexes
• ISSN: 0003-2700; 1520-6882
• DOI: 10.1021/ac971043+

Indirect laser-induced fluorescence was used for the detection of several lanthanide species separated by capillary electrophoresis. Quinine sulfate was the fluorescent component of the background electrolyte, and α-hydroxyisobutyric acid was added as a complexing agent to enable the separation of analyte ions that have similar mobilities. The UV lines (333−364 nm) of an argon ion laser were used as the excitation source with a diode array detector for monitoring the fluorescent emission at 442 nm. Electrokinetic injections and transient isotachophoresis were implemented to stack the analyte ions into more concentrated zones. On-line preconcentration factors were determined to be ∼700 and resulted in limits of detection for La3+, Ce3+, Pr3+, Nd3+, Sm3+, and Eu3+ in the low-ppb range (6−11 nM).