Optically Modulated Fluorophores for Selective Fluorescence Signal Recovery

Fluorescence imaging in biological sciences is hindered by significant depth-dependent signal attenuation and highly fluorescent backgrounds. We have developed optically modulated near-IR-emitting few-atom Ag nanodots that are selectively and dynamically photobrightened upon simultaneous excitation...

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Bibliographic Details
Published inJournal of the American Chemical Society Vol. 131; no. 13; pp. 4619 - 4621
Main Authors Richards, Chris I, Hsiang, Jung-Cheng, Senapati, Dulal, Patel, Sandeep, Yu, Junhua, Vosch, Tom, Dickson, Robert M
Format Journal Article
LanguageEnglish
Published United States American Chemical Society 08.04.2009
Subjects
Animals
Carbocyanines - chemistry
Cytological Techniques
Diagnostic Imaging
Fluorescence
Gold - chemistry
Mice
Nanotubes - chemistry
NIH 3T3 Cells
Optics and Photonics
Photochemistry
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Summary:Fluorescence imaging in biological sciences is hindered by significant depth-dependent signal attenuation and highly fluorescent backgrounds. We have developed optically modulated near-IR-emitting few-atom Ag nanodots that are selectively and dynamically photobrightened upon simultaneous excitation with a secondary laser, enabling high-sensitivity image extraction to reveal only the demodulated fluorophores. Image demodulation is demonstrated in high-background environments to extract weak signals from completely obscuring background emission.
ISSN:0002-7863
1520-5126
DOI:10.1021/ja809785s