Analysis of Polyphenols Using Capillary Zone Electrophoresis and HPLC:  Detection of Soy, Lupin, and Pea Protein in Meat Products

• Published in: Journal of agricultural and food chemistry Vol. 47; no. 2; pp. 594 - 602
• Main Authors: Mellenthin, O, Galensa, R
• Format: Journal Article
• Language: English
• Published: Washington, DC American Chemical Society 01.02.1999
• Subjects: Biological and medical sciences; chemical analysis; Chromatography, High Pressure Liquid; detection; electrophoresis; Electrophoresis, Capillary; Fabaceae - chemistry; Flavonoids; Food industries; Fundamental and applied biological sciences. Psychology; Glycine max - chemistry; high performance liquid chromatography; isoflavones; Isoflavones - chemistry; legume protein; lupin protein; Mass Spectrometry; Meat and meat product industries; meat products; Meat Products - analysis; Phenols - analysis; Pisum sativum - chemistry; Plant Proteins - analysis; Plants, Medicinal; Polymers - analysis; polyphenols; rapid methods; Reference Standards; sausages; soy protein; Legumes; Zone electrophoresis; Capillary electrophoresis; HPLC chromatography; Meat product; Lupin; Photodiode; Flavonoid; Isoflavone derivatives; Pea; Soy protein; Polyphenol; Detector; Analysis method; Quality control; Plant protein; Phenols; Diode array
• ISSN: 0021-8561; 1520-5118
• DOI: 10.1021/jf980749h

The analysis of polyphenols, which are characteristic of certain legumes, enables a rapid and sensitive detection of legume proteins in meat products. Separation of specific isoflavones can be achieved by capillary zone electrophoresis (CZE) or high-performance liquid chromatography (HPLC), both coupled with a photodiode array detector (DAD). The use of CZE in the identification process is an appropriate means of rapid screening; the HPLC is less dependent on matrix effects and clearly more sensitive. Additives of soy protein isolates up to 0.1% could be detected in meat products, even in sausages heated to a high temperature or with hydrolyzed soy proteins. A solid-phase extraction procedure with polyamide cartridges has been developed to concentrate polyphenols. A similar detection of lupin protein is possible in principle. In the case of pea protein, a reliable detection was not possible depending on the coincidental appearance of polyphenols as indicating substances. Keywords: Polyphenols; isoflavones; genistein; legume protein; soy; lupin; pea; meat products; capillary electrophoresis; CZE; HPLC; diode array detection; solid-phase extraction