Watsonianone A from Rhodomyrtus tomentosa Fruit Attenuates Respiratory-Syncytial-Virus-Induced Inflammation In Vitro

• Published in: Journal of agricultural and food chemistry Vol. 65; no. 17; pp. 3481 - 3489
• Main Authors: Zhuang, Ling, Chen, Li-Feng, Zhang, Yu-Bo, Liu, Zhong, Xiao, Xu-Hui, Tang, Wei, Wang, Guo-Cai, Song, Wen-Jun, Li, Yao-Lan, Li, Man-Mei
• Format: Journal Article
• Language: English
• Published: United States American Chemical Society 03.05.2017
• Subjects: Cyclohexanones - pharmacology; Fruit - chemistry; Humans; I-kappa B Proteins - genetics; I-kappa B Proteins - immunology; Interleukin-6 - genetics; Interleukin-6 - immunology; Myrtaceae - chemistry; NF-kappa B - genetics; NF-kappa B - immunology; Plant Extracts - pharmacology; Reactive Oxygen Species - immunology; Respiratory Syncytial Virus Infections - drug therapy; Respiratory Syncytial Virus Infections - immunology; Respiratory Syncytial Virus Infections - virology; Respiratory Syncytial Viruses - drug effects; Respiratory Syncytial Viruses - physiology; Tumor Necrosis Factor-alpha - genetics; Tumor Necrosis Factor-alpha - immunology; watsonianone A; inflammation; respiratory syncytial virus; Rhodomyrtus tomentosa; ROS
• ISSN: 0021-8561; 1520-5118
• DOI: 10.1021/acs.jafc.7b00537

Respiratory syncytial virus (RSV) is one of the most common respiratory pathogens. Immoderate inflammation plays a great role in causing RSV-induced diseases. In the present study, watsonianone A, isolated from the fruit of Rhodomyrtus tomentosa (Ait.) Hassk, was found to show a good inhibitory effect on RSV-induced NO production, with a half-maximal inhibitory concentration of 37.2 ± 1.6 μM. Enzyme-linked immunosorbent assay and fluorescence quantitative polymerase chain reaction analyses indicated that watsonianone A markedly reduced both mRNA and protein levels of tumor necrosis factor α, interleukin 6, and monocyte chemoattractant protein 1 in RSV-infected RAW264.7 cells. Mechanistically, watsonianone A inhibited nuclear factor κB (NF-κB) activation by suppressing IκBα phosphorylation. Further analysis revealed that watsonianone A activated the thioredoxin system and decreased intracellular reactive oxygen species (ROS) levels, which are closely associated with NF-κB activation in RSV-infected cells. These results reveal that watsonianone A can attenuate RSV-induced inflammation via the suppression of ROS-sensitive inflammatory signaling.