Enzymatic Process for High-Yield Turanose Production and Its Potential Property as an Adipogenesis Regulator

• Published in: Journal of agricultural and food chemistry Vol. 64; no. 23; pp. 4758 - 4764
• Main Authors: Park, Min-Oh, Lee, Byung-Hoo, Lim, Eunjin, Lim, Ji Ye, Kim, Yuri, Park, Cheon-Seok, Lee, Hyeon Gyu, Kang, Hee-Kwon, Yoo, Sang-Ho
• Format: Journal Article
• Language: English
• Published: United States American Chemical Society 15.06.2016
• Subjects: 3T3-L1 Cells - drug effects; 3T3-L1 Cells - metabolism; Adipogenesis - drug effects; Animals; Biotechnology - methods; Disaccharides - biosynthesis; Disaccharides - metabolism; Disaccharides - pharmacology; fas Receptor - genetics; Fructose - metabolism; Gene Expression Regulation - drug effects; Glucosyltransferases - chemistry; Glucosyltransferases - metabolism; Lipid Metabolism - drug effects; Mice; PPAR gamma - genetics; Sterol Regulatory Element Binding Protein 1 - genetics; Sucrose - metabolism; amylosucrase; fructose; Neisseria polysaccharea; adipogenesis; turanose
• ISSN: 0021-8561; 1520-5118
• DOI: 10.1021/acs.jafc.5b05849

Turanose is a sucrose isomer naturally existing in honey and a promising functional sweetener due to its low glycemic response. In this study, the extrinsic fructose effect on turanose productivity was examined in Neisseria amylosucrase reaction. Turanose was produced, by increasing the amount of extrinsic fructose as a reaction modulator, with high concentration of sucrose substrate, which resulted in 73.7% of production yield. In physiological functionality test, lipid accumulation in 3T3-L1 preadipocytes in the presence of high amounts of pure glucose was attenuated by turanose substitution in a dose-dependent manner. Turanose treatments at concentrations representing 50%, 75%, and 100% of total glucose concentration in cell media significantly reduced lipid accumulation by 18%, 35%, and 72%, respectively, as compared to controls. This result suggested that turanose had a positive role in controlling adipogenesis, and enzymatic process of turanose production has a potential to develop a functional food ingredient for controlling obesity and related chronic diseases.