Ultrasensitive, Multiplex Raman Frequency Shift Immunoassay of Liver Cancer Biomarkers in Physiological Media

Highly sensitive multiplex biomarker detection is critical for the early diagnosis of liver cancer. Here, a surface-enhanced Raman scattering (SERS) frequency-shift immunoassay is developed for detection of liver cancer biomarkers α-fetoprotein and Glypican-3 down to subpicomolar concentrations in s...

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Published inACS nano Vol. 10; no. 1; pp. 871 - 879
Main Authors Tang, Bochong, Wang, Jiaojiao, Hutchison, James A, Ma, Lei, Zhang, Ning, Guo, Hua, Hu, Zhongbo, Li, Min, Zhao, Yuliang
Format Journal Article
LanguageEnglish
Published United States American Chemical Society 26.01.2016
Subjects
alpha-Fetoproteins - analysis
Animals
Antibodies - chemistry
Biomarkers, Tumor - blood
Carcinoma, Hepatocellular - blood
Carcinoma, Hepatocellular - diagnosis
Cattle
Dimethylpolysiloxanes - chemistry
Early Diagnosis
Glypicans - blood
Humans
Immunoassay
Liver Neoplasms - blood
Liver Neoplasms - diagnosis
Metal Nanoparticles - chemistry
Metal Nanoparticles - ultrastructure
Nylons - chemistry
Printing - methods
Sensitivity and Specificity
Silver - chemistry
Spectrum Analysis, Raman - instrumentation
frequency shift immunoassay
multiplex detection
SERS
liver cancer biomarkers
microcontact printing (μCP)
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Abstract Highly sensitive multiplex biomarker detection is critical for the early diagnosis of liver cancer. Here, a surface-enhanced Raman scattering (SERS) frequency-shift immunoassay is developed for detection of liver cancer biomarkers α-fetoprotein and Glypican-3 down to subpicomolar concentrations in saline solution. A high temperature modification of the Tollen’s method affords silver nanoparticle films with excellent SERS response upon which ordered domains of Raman reporters are chemisorbed by microcontact printing. Shifts in the reporters SERS spectrum in response to a bound antibody’s biomarker recognition constitutes the frequency shift assay, exhibiting here exceptional sensitivity and specificity and shown to function in fetal calf serum and in the serum of a patient with hepatocellular carcinoma.
AbstractList Highly sensitive multiplex biomarker detection is critical for the early diagnosis of liver cancer. Here, a surface-enhanced Raman scattering (SERS) frequency-shift immunoassay is developed for detection of liver cancer biomarkers α-fetoprotein and Glypican-3 down to subpicomolar concentrations in saline solution. A high temperature modification of the Tollen's method affords silver nanoparticle films with excellent SERS response upon which ordered domains of Raman reporters are chemisorbed by microcontact printing. Shifts in the reporters SERS spectrum in response to a bound antibody's biomarker recognition constitutes the frequency shift assay, exhibiting here exceptional sensitivity and specificity and shown to function in fetal calf serum and in the serum of a patient with hepatocellular carcinoma.
Author Hu, Zhongbo
Li, Min
Wang, Jiaojiao
Hutchison, James A
Ma, Lei
Zhao, Yuliang
Tang, Bochong
Guo, Hua
Zhang, Ning
AuthorAffiliation University of Strasbourg and CNRS
Department of Cancer Cell Biology
Chinese Academy of Sciences
College of Materials Science and Optoelectronic Technology
ISIS & icFRC
School of Chemistry and Bio21 Institute
University of Melbourne
CAS Key Laboratory for Biomedical Effects of Nanomaterials and Nanosafety, Institute of High Energy Physics
Tianjin Medical University Cancer Institute and Hospital, National Clinical Research Center for Cancer, Key Laboratory of Cancer Prevention and Therapy
University of Chinese Academy of Sciences
AuthorAffiliation_xml – name: CAS Key Laboratory for Biomedical Effects of Nanomaterials and Nanosafety, Institute of High Energy Physics
– name: College of Materials Science and Optoelectronic Technology
– name: Tianjin Medical University Cancer Institute and Hospital, National Clinical Research Center for Cancer, Key Laboratory of Cancer Prevention and Therapy
– name: Chinese Academy of Sciences
– name: School of Chemistry and Bio21 Institute
– name: University of Melbourne
– name: University of Strasbourg and CNRS
– name: University of Chinese Academy of Sciences
– name: ISIS & icFRC
– name: Department of Cancer Cell Biology
Author_xml – sequence: 1
  givenname: Bochong
  surname: Tang
  fullname: Tang, Bochong
– sequence: 2
  givenname: Jiaojiao
  surname: Wang
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BackLink https://www.ncbi.nlm.nih.gov/pubmed/26731464$$D View this record in MEDLINE/PubMed
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Keywords frequency shift immunoassay
multiplex detection
SERS
liver cancer biomarkers
microcontact printing (μCP)
Language English
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Snippet Highly sensitive multiplex biomarker detection is critical for the early diagnosis of liver cancer. Here, a surface-enhanced Raman scattering (SERS)...
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SubjectTerms alpha-Fetoproteins - analysis
Animals
Antibodies - chemistry
Biomarkers, Tumor - blood
Carcinoma, Hepatocellular - blood
Carcinoma, Hepatocellular - diagnosis
Cattle
Dimethylpolysiloxanes - chemistry
Early Diagnosis
Glypicans - blood
Humans
Immunoassay
Liver Neoplasms - blood
Liver Neoplasms - diagnosis
Metal Nanoparticles - chemistry
Metal Nanoparticles - ultrastructure
Nylons - chemistry
Printing - methods
Sensitivity and Specificity
Silver - chemistry
Spectrum Analysis, Raman - instrumentation
Title Ultrasensitive, Multiplex Raman Frequency Shift Immunoassay of Liver Cancer Biomarkers in Physiological Media
URI http://dx.doi.org/10.1021/acsnano.5b06007
https://www.ncbi.nlm.nih.gov/pubmed/26731464
https://search.proquest.com/docview/1760924707
Volume 10
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