Ultrasensitive, Multiplex Raman Frequency Shift Immunoassay of Liver Cancer Biomarkers in Physiological Media

• Published in: ACS nano Vol. 10; no. 1; pp. 871 - 879
• Main Authors: Tang, Bochong, Wang, Jiaojiao, Hutchison, James A, Ma, Lei, Zhang, Ning, Guo, Hua, Hu, Zhongbo, Li, Min, Zhao, Yuliang
• Format: Journal Article
• Language: English
• Published: United States American Chemical Society 26.01.2016
• Subjects: alpha-Fetoproteins - analysis; Animals; Antibodies - chemistry; Biomarkers, Tumor - blood; Carcinoma, Hepatocellular - blood; Carcinoma, Hepatocellular - diagnosis; Cattle; Dimethylpolysiloxanes - chemistry; Early Diagnosis; Glypicans - blood; Humans; Immunoassay; Liver Neoplasms - blood; Liver Neoplasms - diagnosis; Metal Nanoparticles - chemistry; Metal Nanoparticles - ultrastructure; Nylons - chemistry; Printing - methods; Sensitivity and Specificity; Silver - chemistry; Spectrum Analysis, Raman - instrumentation; frequency shift immunoassay; multiplex detection; SERS; liver cancer biomarkers; microcontact printing (μCP)
• ISSN: 1936-0851; 1936-086X
• DOI: 10.1021/acsnano.5b06007

Highly sensitive multiplex biomarker detection is critical for the early diagnosis of liver cancer. Here, a surface-enhanced Raman scattering (SERS) frequency-shift immunoassay is developed for detection of liver cancer biomarkers α-fetoprotein and Glypican-3 down to subpicomolar concentrations in saline solution. A high temperature modification of the Tollen’s method affords silver nanoparticle films with excellent SERS response upon which ordered domains of Raman reporters are chemisorbed by microcontact printing. Shifts in the reporters SERS spectrum in response to a bound antibody’s biomarker recognition constitutes the frequency shift assay, exhibiting here exceptional sensitivity and specificity and shown to function in fetal calf serum and in the serum of a patient with hepatocellular carcinoma.