Knee Joint Injection Resveratrol Amelioration Inflammation in Collagen Antibody Induced Arthritis

• Published in: Journal of Medical Sciences Vol. 39; no. 5; pp. 223 - 230
• Main Authors: Tseng, Feng-Jen, Lin, Gu-Jiun, Chen, Yuan-Wu, Lee, Herng-Sheng, Sytwu, Huey-Kang, Chia, Wei-Tso
• Format: Journal Article
• Language: English
• Published: 國防醫學院 01.09.2019; Medknow Publications and Media Pvt. Ltd
• Subjects: Anti-inflammatory agents; Antibodies; Arthritis; Collagen; Dimethyl sulfoxide; Enzymes; Gene expression; Health aspects; Inflammation; Laboratory rats; Medical research; Resveratrol; Risk factors; anti-inflammation; experimental arthritis; knee joint injection; Resveratrol
• ISSN: 1011-4564
• DOI: 10.4103/jmedsci.jmedsci_8_19

Background: Resveratrol has been considered to possess anti-inflammatory properties. Here, we propose that intra-articular resveratrol injection might ameliorate acute inflammation in a single knee joint in mice with collagen antibody-induced arthritis (CAIA). Methods: The clinical expression of CAIA was analyzed by two investigators. Resveratrol (30 mg/kg in 30 μL dimethyl sulfoxide [DMSO]) was injected into the knee joint of BALB/c mice from days 0 to 9. Footpad thickness was photographed and calculated. On day 10, the joints were examined histopathologically. Serum cytokine levels were measured by enzyme-linked immunosorbent assay. mRNA levels in paws were measured by real-time polymerase chain reaction (PCR). Results: In CAIA, acute inflammation was induced on day 7 and continued until day 10. In the resveratrol-treated mice, the severity of inflammation was obviously reduced. Expression levels of interleukin-1β, cyclooxygenase-2 (COX-2), and inducible nitric oxide synthase were decreased in the treated mice; however, tumor necrosis factor-α (TNF-α) levels did not change significantly after treatment. Conclusion: The anti-inflammatory mechanism of resveratrol is associated with NF-κB inhibition. Further investigations of the clinical applications of resveratrol are required.