Cationic Phosphorus Dendrimer Enhances Photodynamic Activity of Rose Bengal against Basal Cell Carcinoma Cell Lines

In the last couple of decades, photodynamic therapy emerged as a useful tool in the treatment of basal cell carcinoma. However, it still meets limitations due to unfavorable properties of photosensitizers such as poor solubility or lack of selectivity. Dendrimers, polymers widely studied in biomedic...

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Published inMolecular pharmaceutics Vol. 14; no. 5; pp. 1821 - 1830
Main Authors Dabrzalska, Monika, Janaszewska, Anna, Zablocka, Maria, Mignani, Serge, Majoral, Jean Pierre, Klajnert-Maculewicz, Barbara
Format Journal Article
LanguageEnglish
Published United States American Chemical Society 01.05.2017
Subjects
Animals
Cell Line, Tumor
Chemical Sciences
Coordination chemistry
Dendrimers - chemistry
Mice
Phosphorus - chemistry
Photochemotherapy - methods
Photosensitizing Agents - chemistry
Photosensitizing Agents - therapeutic use
Reactive Oxygen Species - metabolism
Rose Bengal - chemistry
rose bengal
basal cell carcinoma
phosphorus dendrimer
phototoxicity
photodynamic therapy
photosensitizer
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Summary:In the last couple of decades, photodynamic therapy emerged as a useful tool in the treatment of basal cell carcinoma. However, it still meets limitations due to unfavorable properties of photosensitizers such as poor solubility or lack of selectivity. Dendrimers, polymers widely studied in biomedical field, may play a role as photosensitizer carriers and improve the efficacy of photodynamic treatment. Here, we describe the evaluation of an electrostatic complex of cationic phosphorus dendrimer and rose bengal in such aspects as singlet oxygen production, cellular uptake, and phototoxicity against three basal cell carcinoma cell lines. Rose bengal–cationic dendrimer complex in molar ratio 5:1 was compared to free rose bengal. Obtained results showed that the singlet oxygen production in aqueous medium was significantly higher for the complex than for free rose bengal. The cellular uptake of the complex was 2–7-fold higher compared to a free photosensitizer. Importantly, rose bengal, rose bengal–dendrimer complex, and dendrimer itself showed no dark toxicity against all three cell lines. Moreover, we observed that phototoxicity of the complex was remarkably enhanced presumably due to high cellular uptake. On the basis of the obtained results, we conclude that rose bengal–cationic dendrimer complex has a potential in photodynamic treatment of basal cell carcinoma.
ISSN:1543-8384
1543-8392
DOI:10.1021/acs.molpharmaceut.7b00108