Self-Assembled N‑Heterocyclic Carbene-Based Carboxymethylated Dextran Monolayers on Gold as a Tunable Platform for Designing Affinity-Capture Biosensor Surfaces

• Published in: ACS applied materials & interfaces Vol. 10; no. 21; pp. 17560 - 17570
• Main Authors: Li, Zhijun, Munro, Kim, Narouz, Mina R, Lau, Andrew, Hao, Hongxia, Crudden, Cathleen M, Horton, J. Hugh
• Format: Journal Article
• Language: English
• Published: United States American Chemical Society 30.05.2018
• Subjects: Biosensing Techniques; Dextrans; Gold; Methane - analogs & derivatives; streptavidin; nitrilotriacetic acid; carboxymethylated dextran; surface plasmon resonance; biosensing; protein A; NHCs; N-heterocyclic carbenes
• ISSN: 1944-8244; 1944-8252
• DOI: 10.1021/acsami.8b02595

Sensor surfaces play a predominant role in the development of optical biosensor technologies for the analysis of biomolecular interactions. Thiol-based self-assembled monolayers (SAMs) on gold have been widely used as linker layers for sensor surfaces. However, the degradation of the thiol–gold bond can limit the performance and durability of such surfaces, directly impacting their performance and cost-effectiveness. To this end, a new family of materials based on N-heterocyclic carbenes (NHCs) has emerged as an alternative for surface modification, capable of self-assembling onto a gold surface with higher affinity and superior stability as compared to the thiol-based systems. Here we demonstrate three applications of NHC SAMs supporting a dextran layer as a tunable platform for developing various affinity-capture biosensor surfaces. We describe the development and testing of NHC-based dextran biosensor surfaces modified with each of streptavidin, nitrilotriacetic acid, and recombinant Protein A. These affinity-capture sensor surfaces enable oriented binding of ligands for optimal performance in biomolecular assays. Together, the intrinsic high stability and flexible design of the NHC biosensing platforms show great promise and open up exciting possibilities for future biosensing applications.