Fluorescent Structural DNA Nanoballs Functionalized with Phosphate-Linked Nucleotide Triphosphates

• Published in: Nano letters Vol. 10; no. 3; pp. 788 - 792
• Main Authors: Anderson, Jon P, Reynolds, Bambi L, Baum, Kristin, Williams, John G
• Format: Journal Article
• Language: English
• Published: Washington, DC American Chemical Society 10.03.2010
• Subjects: Chemical synthesis methods; Cross-disciplinary physics: materials science; rheology; Cross-Linking Reagents - chemistry; Crystallization - methods; DNA - chemistry; DNA - ultrastructure; Exact sciences and technology; Fluorescent Dyes - chemistry; Macromolecular Substances - chemistry; Materials science; Materials Testing; Methods of nanofabrication; Molecular Conformation; Nanostructures - chemistry; Nanostructures - ultrastructure; Nanotechnology - methods; Nucleotides - chemistry; Particle Size; Phosphates - chemistry; Physics; SYBR; Single molecule sequencing; DNA nanoball; phosphate-labeled nucleotide; fluorescent; Phosphates; Atomic force microscopy; Fluorescent material; Nanoball; DNA; Polymerization; Signal-to-noise ratio; Template reaction; Fluorescence microscopy
• ISSN: 1530-6984; 1530-6992
• DOI: 10.1021/nl9039718

Highly labeled DNA nanoballs functionalized with phosphate-linked nucleotide triphosphates (dNTPs) were developed as a source of dNTPs for DNA polymerase. The particles were prepared by strand-displacement polymerization from a self-complementary circular template. Imaged by atomic force microscopy, these functionalized particles appear as condensed fuzzy balls with diameters between 50 and 150 nm. They emit a bright fluorescent signal, detected in 2 ms exposures with a signal-to-noise ratio of 25 when imaged using a TIR fluorescence microscope.