Penguin-Driven Dissemination and High Enrichment of Antibiotic Resistance Genes in Lake Sediments across Antarctica

• Published in: Environmental science & technology Vol. 58; no. 32; pp. 14460 - 14474
• Main Authors: Zhou, Zeming, Zhu, Renbin, Song, Yixin, Zhang, Wanying, Sun, Bowen, Zhang, Zulin, Yao, Huaiying
• Format: Journal Article
• Language: English
• Published: United States American Chemical Society 13.08.2024
• Subjects: Abundance; animal manures; Antarctica; Antibiotic resistance; Antibiotics; bacterial communities; Deposition; Drug resistance; Dung; Environmental factors; estuaries; Food chains; Genes; Genetic analysis; Guano; Lake sediments; Lakes; Occurrence, Fate, and Transport of Aquatic and Terrestrial Contaminants; Penguins; pollution; Redundancy; Resistance factors; Sediment samplers; Sediments; swine; technology; Antarctica; mobile genetic elements; lake sediments; Antarctica; bacterial community; antibiotic resistance genes; penguin
• ISSN: 0013-936X; 1520-5851; 1520-5851
• DOI: 10.1021/acs.est.4c02732

Numerous penguins can propagate pathogens with antibiotic resistance genes (ARGs) into Antarctica. However, the effects of penguin dissemination on the lake ARGs still have received little attention via guano deposition. Here, we have profiled ARGs in ornithogenic sediments subject to penguin guano (OLS) and nonornithogenic sediments (NOLS) from 16 lakes across Antarctica. A total of 191 ARGs were detected in all sediment samples, with a much higher abundance and diversity in OLS than in NOLS. Surprisingly, highly diverse and abundant ARGs were found in the OLS with a detection frequency of >40% and an absolute abundance of (2.34 × 109)–(4.98 × 109) copies g–1, comparable to those in coastal estuarine sediments and pig farms. The strong correlations of identified resistance genes with penguin guano input amount, environmental factors, mobile genetic elements, and bacterial community, in conjunction with network and redundancy analyses, all indicated that penguins were responsible for the dissemination and high enrichment of ARGs in lake sediments via the guano deposition, which might greatly outweigh local human-activity effects. Our results revealed that ARGs could be carried into lakes across the Antarctica through penguin migration, food chains, and guano deposition, which were closely connected with the widespread pollution of ARGs at the global scale.