Anaerobic Degradation of Aromatic and Aliphatic Biodegradable Plastics: Potential Mechanisms and Pathways

• Published in: Environmental science & technology Vol. 58; no. 43; pp. 19462 - 19474
• Main Authors: Zhang, Yuchen, Wang, Zijiang, Wang, Feng, Zhou, Hansheng, Zhang, Liangmao, Xie, Bing
• Format: Journal Article
• Language: English
• Published: United States American Chemical Society 29.10.2024
• Subjects: Acetic acid; Acetogenesis; Aerobic conditions; Anaerobic biodegradation; Anaerobic conditions; Anaerobic digestion; Anaerobic microorganisms; Biodegradability; Biodegradable materials; Biodegradation; Bioplastics; Carbon dioxide; carbon dioxide production; Degradability; Degradation; Depolymerization; Environmental degradation; Environmental engineering; environmental fate; enzymes; Hydrolysis; Metagenomics; Methane; Microbial degradation; Microorganisms; Molecular weight; Occurrence, Fate, and Transport of Aquatic and Terrestrial Contaminants; Plastics; Polymer blends; Polymers; Thermophilic bacteria; Thermophilic microorganisms; Biodegradable microplastics; PLA; Plastic-degrading microorganisms; PABT; Anaerobic digestion
• ISSN: 0013-936X; 1520-5851; 1520-5851
• DOI: 10.1021/acs.est.4c07554

Biodegradable plastics (BDPs) have been widely used as substitutes for traditional plastics, and their environmental fate is a subject of intense research interest. Compared with the aerobic degradation of BDPs, their biodegradability under anaerobic conditions in environmental engineering systems remains poorly understood. This study aimed to investigate the degradability of BDPs composed of poly­(butylene adipate-co-terephthalate) (PBAT), poly­(lactide acid) (PLA), and their blends, and explore the mechanism underlying their microbial degradation under conditions of anaerobic digestion (AD). The BDPs readily depolymerized under thermophilic conditions but were hydrolyzed at a slow rate under conditions of mesophilic AD. After 45 days of thermophilic AD, a decrease in the molecular weight and significant increase in the production of methane and carbon dioxide production were observed. Network and metagenomics analyses identified AD as reservoirs of plastic-degrading bacteria that produce multiple plastic-degrading enzymes. PETase was identified as the most abundant plastic-degrading enzyme. A potential pathway for the anaerobic biodegradation of BDPs was proposed herein. The polymers of high molecular weight were subjected to abiotic hydrolysis to form oligomers and monomers, enabling subsequent microbial hydrolysis and acetogenesis. Ultimately, complete degradation was achieved predominantly via the pathway involved in the conversion of acetic acid to methane. These findings provide novel insight into the mechanism underlying the anaerobic degradation of BDPs and the microbial resources crucial for the efficient degradation of BDPs.