Combination of Two Synchrotron Radiation-Based Techniques and Chemometrics to Study an Enhanced Natural Remineralization of Enamel

The limitations to assess dental enamel remineralization have been overcome by a methodology resulting from the appropriate combination of synchrotron radiation-based techniques on both, infrared microspectroscopy and micro X-ray diffraction, with the help of specific data mining. Since amelogenin p...

Full description

Saved in:
Bibliographic Details
Published inAnalytical chemistry (Washington) Vol. 94; no. 13; pp. 5359 - 5366
Main Authors Diez-García, Sandra, Sánchez-Martín, María-Jesús, Amigo, José Manuel, Valiente, Manuel
Format Journal Article
LanguageEnglish
Published United States American Chemical Society 05.04.2022
Subjects
Amelogenin
Analytical chemistry
Calcium ions
Chemistry
Chemometrics
Crystal growth
Data mining
Dental Enamel
Dental restorative materials
Enamel
Fluorapatite
Impurities
Ion exchange
Ion exchange resins
Ions
Mineralization
Principal components analysis
Radiation
Remineralization
Resins
Synchrotron radiation
Synchrotrons
Teeth
Tooth Remineralization
X-Ray Diffraction
Zinc
Online AccessGet full text

Cover

More Information
Summary:The limitations to assess dental enamel remineralization have been overcome by a methodology resulting from the appropriate combination of synchrotron radiation-based techniques on both, infrared microspectroscopy and micro X-ray diffraction, with the help of specific data mining. Since amelogenin plays a key role in modulating the mineralization of tooth enamel, we propose a controlled ion release for fluorapatite structural ions (Ca2+, PO4 3–, and F–, also including Zn2+) by using weak acid and weak base ion-exchange resins in the presence of amelogenin to remineralize the surface of etched teeth. This combination provides the necessary ions for enamel remineralization and a guide for crystal growth due to the protein. Remineralized tooth samples were analyzed by applying the indicated methodology. The synchrotron data were treated using principal component analysis and multivariate curve resolution to analyze the mineral layer formed in the presence and absence of amelogenin. The remineralizing treatment created a fluorapatite layer free of carbonate impurities and with a similar orientation to that of the natural enamel thanks to amelogenin contribution.
Bibliography:ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
ISSN:0003-2700
1520-6882
DOI:10.1021/acs.analchem.1c05498