Isolation of DNA from saliva of betel quid chewers using treated cards

Betel quid (BQ) chewing, a common tradition in tropical areas, often poses a problem during collection and DNA analysis of buccal samples from many indigenous communities for population genetic studies and in forensic analysis of chewed BQ residues. This study evaluated the use of FTA card, a chemic...

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Bibliographic Details
Published inJournal of forensic sciences Vol. 48; no. 4; p. 794
Main Authors Salvador, Jazelyn M, De Ungria, Maria Corazon A
Format Journal Article
LanguageEnglish
Published United States 01.07.2003
Subjects
Areca - chemistry
Case-Control Studies
DNA - isolation & purification
DNA Fingerprinting - instrumentation
DNA Fingerprinting - methods
Genotype
Humans
Polymerase Chain Reaction
Saliva - physiology
Tandem Repeat Sequences
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Summary:Betel quid (BQ) chewing, a common tradition in tropical areas, often poses a problem during collection and DNA analysis of buccal samples from many indigenous communities for population genetic studies and in forensic analysis of chewed BQ residues. This study evaluated the use of FTA card, a chemically treated filter paper, in collecting buccal samples from long-term BQ users and subsequent PCR-based analysis using nine STR markers. A low overall success rate of amplification was observed in the samples extracted using a standard organic extraction procedure (7%) as compared with those prepared using the FTA card (89%). The presence of inhibitors in liquid DNA samples was verified when control DNA failed to amplify in the presence of an equal volume of liquid BQ samples. The use of the FTA card is more practical during field sampling than handling tubes containing buccal swabs.
ISSN:0022-1198
DOI:10.1520/JFS2002347