miRNA Detection for Prostate Cancer Diagnosis by miRoll-Cas: miRNA Rolling Circle Transcription for CRISPR-Cas Assay

Micro-RNA (miRNA) emerges as a promising type of biomarker for cancer diagnosis. There is an urgent need for developing rapid, convenient, and precise miRNA detection methods that may be conducted with limited laboratory facilities, especially in underdeveloped areas. Herein, we developed a miRNA de...

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Bibliographic Details
Published inAnalytical chemistry (Washington) Vol. 95; no. 35; pp. 13220 - 13226
Main Authors Ma, Xiaowei, Zhou, Fei, Yang, Donglei, Chen, Yun, Li, Min, Wang, Pengfei
Format Journal Article
LanguageEnglish
Published Washington American Chemical Society 05.09.2023
Subjects
analytical chemistry
Biomarkers
Chemistry
CRISPR
CRISPR-Cas systems
Diagnosis
microRNA
miRNA
people
Prostate cancer
prostatic neoplasms
Ribonucleic acid
RNA
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Summary:Micro-RNA (miRNA) emerges as a promising type of biomarker for cancer diagnosis. There is an urgent need for developing rapid, convenient, and precise miRNA detection methods that may be conducted with limited laboratory facilities, especially in underdeveloped areas. Herein, we developed a miRNA detection method termed miRoll-Cas, where miRNA is first amplified by rolling circle transcription and then subject to CRISPR-Cas13a assay. Using miRoll-Cas, we realized the sensitive detection of multiple cancer-relevant miRNA markers (miR21, miR141, and Let7b) and specifically identified other variants of the Let7 family, which can accurately discriminate prostate cancer patients from healthy people. We envision that miRoll-Cas may be readily translated to clinical applications in the diagnosis of a variety of diseases beyond cancer.
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ISSN:0003-2700
1520-6882
1520-6882
DOI:10.1021/acs.analchem.3c02231