Active site directed inactivation of rat mammary gland fatty acid synthase by 3-chloropropionyl coenzyme A

• Published in: Biochemistry (Easton) Vol. 25; no. 2; pp. 468 - 473
• Main Authors: Miziorko, Henry M, Behnke, Christine E, Ahmad, Patricia M, Ahmad, Fazal
• Format: Journal Article
• Language: English
• Published: United States American Chemical Society 28.01.1986
• Subjects: 3-chloropropionyl coenzyme A; Acyl Coenzyme A - chemical synthesis; Acyl Coenzyme A - pharmacology; Animals; Binding Sites; fatty acid synthase; Fatty Acid Synthases - antagonists & inhibitors; Fatty Acid Synthases - isolation & purification; Female; Kinetics; mammary gland; Mammary Glands, Animal - enzymology; Molecular Weight; Rats
• ISSN: 0006-2960; 1520-4995
• DOI: 10.1021/bi00350a029

3-Chloropropionyl coenzyme A (CoA) irreversibly inhibits rat mammary gland fatty acid synthase. Enzyme inactivation proceeds with first-order kinetics. NADPH (150 microM) as well as acetyl-CoA (500 microM) affords protection against inactivation, suggesting that the inhibitor is active site directed. In contrast, malonyl-CoA (500 microM) offers little protection. With chloro [1-14C]propionyl-CoA, stoichiometries of modification that approach one per enzyme protomer (240 kilodaltons) have been measured. When chloropropionyl-[3'-32P]CoA is used for inactivation, modification stoichiometries are less than 10% of the value observed in the 14C labeling experiments, suggesting that acylation of the enzyme occurs. Radioactivity remains associated with the 14C-labeled protein after performic acid oxidation, indicating that another linkage, in addition to the thio ester adduct, is formed during inactivation. Recovery of [( 14C]carboxyethyl)cysteine from digests of the inactivated enzyme indicates that alkylation of an active site cysteine occurs. The cysteamine sulfhydryl of the acyl carrier peptide is clearly not the site of modification. Loss of overall enzyme activity is tightly linked to decreases in the ketoacyl synthase partial reaction. This observation, coupled with the differential protection measured with acetyl-CoA and malonyl-CoA, suggests that the reagent modifies a residue at the active site involved in condensation. While inactivated enzyme shows good ketoacyl reductase activity when S-(acetoacetyl)-N-acetylcysteamine is used as a substrate, only poor activity for this partial reaction is measured when acetoacetyl-CoA is the substrate. This implies that the function of the acyl carrier peptide (ACP) is impaired during the inactivation process.