A Portable Magnetic Particle Spectrometer for Future Rapid and Wash-Free Bioassays

• Published in: ACS applied materials & interfaces Vol. 13; no. 7; pp. 7966 - 7976
• Main Authors: Wu, Kai, Chugh, Vinit Kumar, di Girolamo, Arturo, Liu, Jinming, Saha, Renata, Su, Diqing, Krishna, Venkatramana D, Nair, Abilash, Davies, Will, Wang, Yongqiang Andrew, Cheeran, Maxim C-J, Wang, Jian-Ping
• Format: Journal Article
• Language: English
• Published: United States American Chemical Society 24.02.2021
• Subjects: Biological and Medical Applications of Materials and Interfaces; Biological Assay - instrumentation; COVID-19 - diagnosis; Humans; Hydrodynamics; Influenza, Human - diagnosis; Magnetic Phenomena; Magnetite Nanoparticles - chemistry; Particle Size; Smartphone; Streptavidin - analysis; Surface Properties; bioassay; wash-free; portable; point-of-care; magnetic particle spectrometer
• ISSN: 1944-8244; 1944-8252
• DOI: 10.1021/acsami.0c21040

Nowadays, there is an increasing demand for more accessible routine diagnostics for patients with respect to high accuracy, ease of use, and low cost. However, the quantitative and high accuracy bioassays in large hospitals and laboratories usually require trained technicians and equipment that is both bulky and expensive. In addition, the multistep bioassays and long turnaround time could severely affect the disease surveillance and control especially in pandemics such as influenza and COVID-19. In view of this, a portable, quantitative bioassay device will be valuable in regions with scarce medical resources and help relieve burden on local healthcare systems. Herein, we introduce the MagiCoil diagnostic device, an inexpensive, portable, quantitative, and rapid bioassay platform based on the magnetic particle spectrometer (MPS) technique. MPS detects the dynamic magnetic responses of magnetic nanoparticles (MNPs) and uses the harmonics from oscillating MNPs as metrics for sensitive and quantitative bioassays. This device does not require trained technicians to operate and employs a fully automatic, one-step, and wash-free assay with a user friendly smartphone interface. Using a streptavidin-biotin binding system as a model, we show that the detection limit of the current portable device for streptavidin is 64 nM (equal to 5.12 pmole). In addition, this MPS technique is very versatile and allows for the detection of different diseases just by changing the surface modifications on MNPs. Although MPS-based bioassays show high sensitivities as reported in many literatures, at the current stage, this portable device faces insufficient sensitivity and needs further improvements. It is foreseen that this kind of portable device can transform the multistep, laboratory-based bioassays to one-step field testing in nonclinical settings such as schools, homes, offices, etc.