Preparation of a Branched DNA Self-Assembled Monolayer toward Sensitive DNA Biosensors

To realize an efficient hybridization of oligonucleotides, a DNA self-assembled monolayer (SAM) composed of branched probe DNA containing single- and double-stranded portions was prepared on a gold substrate. Hybridization using the SAM was monitored in situ by surface plasmon resonance (SPR). By co...

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Bibliographic Details
Published inNano letters Vol. 3; no. 8; pp. 1083 - 1086
Main Authors Nakamura, Fumio, Ito, Eisuke, Sakao, Yosuke, Ueno, Nobuo, Gatuna, Isaiah N, Ohuchi, Fumio S, Hara, Masahiko
Format Journal Article
LanguageEnglish
Published Washington, DC American Chemical Society 01.08.2003
Subjects
Biological and medical sciences
Biosensors
Biotechnology
Fundamental and applied biological sciences. Psychology
Methods. Procedures. Technologies
Various methods and equipments
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Summary:To realize an efficient hybridization of oligonucleotides, a DNA self-assembled monolayer (SAM) composed of branched probe DNA containing single- and double-stranded portions was prepared on a gold substrate. Hybridization using the SAM was monitored in situ by surface plasmon resonance (SPR). By controlling the surface coverage of the probe DNA, hybridization efficiency was optimized. SPR results strongly indicate that all probe sites are accessible for hybridization under optimized conditions regardless of the position.
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ISSN:1530-6984
1530-6992
DOI:10.1021/nl0342794