Role of Ionic Strength in Staphylococcal Cell Aggregation

• Published in: Langmuir Vol. 32; no. 29; pp. 7277 - 7283
• Main Authors: Vanzieleghem, Thomas, Couniot, Numa, Herman-Bausier, Philippe, Flandre, Denis, Dufrêne, Yves F, Mahillon, Jacques
• Format: Journal Article
• Language: English
• Published: United States American Chemical Society 26.07.2016
• Subjects: Interfaces: Adsorption, Reactions, Films, Forces, Measurement Techniques, Charge Transfer, Electrochemistry, Electrocatalysis, Energy Production and Storage; Microscopy, Atomic Force; Osmolar Concentration; Staphylococcus - chemistry; Staphylococcus - ultrastructure
• ISSN: 0743-7463; 1520-5827
• DOI: 10.1021/acs.langmuir.6b00499

Cell aggregation plays a key role in biofilm formation and pathogenesis of Staphylococcus species. Although the molecular basis of aggregation in Staphylococci has already been extensively investigated, the influence of environmental factors, such as ionic strength, remains poorly understood. In this paper, we report a new type of cellular aggregation of Staphylococci that depends solely on ionic strength. Seven strains out of 14, all belonging to staphylococcal species, formed large cell clusters within minutes in buffers of ionic strength ranging from 1.5 to 50 mM, whereas isolates belonging to other Gram-positive species did not display this phenotype. Atomic force microscopy (AFM) with chemically functionalized tips provided direct evidence that ionic strength modulates cell surface adhesive properties through changes in cell surface charge. The optimal ionic strength for aggregation was found to be strain dependent, but in all cases, bacterial aggregates formed at an ionic strength of 1.5–50 mM were rapidly dispersed in a solution of higher ionic strength, indicating a reversibility of the cell aggregation process. These findings suggest that some staphylococcal isolates can respond to ionic strength as an external stimulus to trigger rapid cell aggregation in a way that has not yet been reported.