Serial Metabolome Changes in a Prospective Cohort of Subjects with Influenza Viral Infection and Comparison with Dengue Fever

• Published in: Journal of proteome research Vol. 16; no. 7; pp. 2614 - 2622
• Main Authors: Cui, Liang, Fang, Jinling, Ooi, Eng Eong, Lee, Yie Hou
• Format: Journal Article
• Language: English
• Published: United States American Chemical Society 07.07.2017
• Subjects: Adult; adults; beta oxidation; bile acids; biosynthesis; cohort studies; dengue; Dengue - blood; Dengue - diagnosis; Dengue - virology; Dengue Virus - pathogenicity; Dengue Virus - physiology; Fatty Acids - blood; Female; fever; Gonadal Steroid Hormones - blood; Gonadal Steroid Hormones - genetics; Host-Pathogen Interactions; Humans; hypoxanthine; influenza; Influenza A Virus, H3N2 Subtype - pathogenicity; Influenza A Virus, H3N2 Subtype - physiology; Influenza, Human - blood; Influenza, Human - diagnosis; Influenza, Human - virology; inosine; linoleate 13S-lipoxygenase; Lipid Metabolism - genetics; Lipoxygenase - blood; Lipoxygenase - genetics; Male; Metabolic Networks and Pathways - genetics; metabolites; Metabolome; metabolomics; Middle Aged; Orthomyxoviridae; Oxidation-Reduction; oxylipins; phospholipids; Phospholipids - blood; Prospective Studies; proteome; public health; Purines - blood; serotonin; steroid hormones; tryptophan; Tryptophan - blood; xanthine; influenza; oxylipins; metabolomics; dengue; H3N2; infectious diseases
• ISSN: 1535-3893; 1535-3907; 1535-3907
• DOI: 10.1021/acs.jproteome.7b00173

Influenza virus infection (IVI) and dengue virus infection (DVI) are major public health threats. Between IVI and DVI, clinical symptoms can be overlapping yet infection-specific, but host metabolome changes are not well-described. Untargeted metabolomics and targeted oxylipinomic analyses were performed on sera serially collected at three phases of infection from a prospective cohort study of adult subjects with either H3N2 influenza infection or dengue fever. Untargeted metabolomics identified 26 differential metabolites, and major perturbed pathways included purine metabolism, fatty acid biosynthesis and β-oxidation, tryptophan metabolism, phospholipid catabolism, and steroid hormone pathway. Alterations in eight oxylipins were associated with the early symptomatic phase of H3N2 flu infection, were mostly arachidonic acid-derived, and were enriched in the lipoxygenase pathway. There was significant overlap in metabolome profiles in both infections. However, differences specific to IVI and DVI were observed. DVI specifically attenuated metabolites including serotonin, bile acids and biliverdin. Additionally, metabolome changes were more persistent in IVI in which metabolites such as hypoxanthine, inosine, and xanthine of the purine metabolism pathway remained significantly elevated at 21–27 days after fever onset. This study revealed the dynamic metabolome changes in IVI subjects and provided biochemical insights on host physiological similarities and differences between IVI and DVI.