Biphasic Decay Kinetics of Fecal Bacteria in Surface Water Not a Density Effect

• Published in: Journal of environmental engineering (New York, N.Y.) Vol. 135; no. 5; pp. 372 - 376
• Main Authors: Hellweger, F. L, Bucci, V, Litman, M. R, Gu, A. Z, Onnis-Hayden, A
• Format: Journal Article
• Language: English
• Published: Reston, VA American Society of Civil Engineers 01.05.2009
• Subjects: Applied sciences; Biological and physicochemical phenomena; Continental surface waters; Escherichia coli; Exact sciences and technology; Natural water pollution; Pollution; TECHNICAL NOTES; Water treatment and pollution; Phosphates; Escherichia coli; Density; Modeling; Hardening; Heterogeneity; Recreation; First order; Surface water; Water quality; Decay; Bacteria; Water pollution; Kinetics; Rate constant; Public health; Enterobacteriaceae
• ISSN: 0733-9372; 1943-7870
• DOI: 10.1061/(ASCE)0733-9372(2009)135:5(372)

The decay of fecal bacteria in surface water often follows a biphasic pattern with the apparent first-order rate constant relatively high during a first phase and lower in a second one. This could be the result of population heterogeneity in resistance due to various mechanisms (different strains, genetically or nongenetically differentiated cells, different growth or cell cycle stage, clumping, hardening), or the specific decay rate could be directly or indirectly affected by the cell density (e.g., quorum sensing). All these mechanisms can theoretically produce a biphasic decay pattern and are consistent with the literature. However, they are fundamentally different and lead to different behavior of mechanistic total maximum daily load models, so identifying the correct mechanism is important. This technical note presents the results of a study aimed at determining if a density effect is involved. Laboratory decay experiments with pure strain Escherichia coli cells in phosphate buffer were conducted over a range of initial densities. The results show that the rate constant changes after a certain time, rather than at a certain density, which is inconsistent with a density effect. As the experiments were performed with a pure strain, the resistant fraction cannot be attributed to a different strain. Further research is needed to identify the mechanism responsible for the population heterogeneity.