Effect of Heparin on Viologen-Stimulated Enzymatic NADH Depletion

Paraquat and diquat undergo redox cycling mediated by xanthine oxidase in the NADH-dependent manner. In these processes, the rates of NADH oxidation and superoxide formation are increased almost 10-fold. The addition of heparin can substantially inhibit these processes. A protective role of heparin...

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Bibliographic Details
Published inChemical research in toxicology Vol. 19; no. 5; pp. 668 - 673
Main Authors Zielonka, Jacek, Rybak, Małgorzata, Celińska, Joanna, Adamus, Jan, Marcinek, Andrzej, Gebicki, Jerzy
Format Journal Article
LanguageEnglish
Published United States American Chemical Society 01.05.2006
Subjects
Cytochrome c Group - chemistry
Diquat - chemistry
Heparin - chemistry
NADP - chemistry
Oxidation-Reduction
Paraquat - chemistry
Superoxides - chemistry
Xanthine Oxidase - chemistry
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Summary:Paraquat and diquat undergo redox cycling mediated by xanthine oxidase in the NADH-dependent manner. In these processes, the rates of NADH oxidation and superoxide formation are increased almost 10-fold. The addition of heparin can substantially inhibit these processes. A protective role of heparin against oxygen radicals formation can be rationalized in terms of its ability to bind paraquat or diquat. The binding process has been investigated by means of the pulse radiolysis technique. Biological consequences of the binding processes are discussed.
Bibliography:istex:21D9A6240F27095483F7A93BE9B40184CAB884E5
ark:/67375/TPS-QFJ3HR4K-B
ObjectType-Article-1
SourceType-Scholarly Journals-1
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ISSN:0893-228X
1520-5010
DOI:10.1021/tx050336s