Polyketide Bioderivatization Using the Promiscuous Acyltransferase KirCII

• Published in: ACS synthetic biology Vol. 6; no. 3; pp. 421 - 427
• Main Authors: Musiol-Kroll, Ewa M, Zubeil, Florian, Schafhauser, Thomas, Härtner, Thomas, Kulik, Andreas, McArthur, John, Koryakina, Irina, Wohlleben, Wolfgang, Grond, Stephanie, Williams, Gavin J, Lee, Sang Yup, Weber, Tilmann
• Format: Journal Article
• Language: English
• Published: United States American Chemical Society 17.03.2017
• Subjects: Acyltransferases - metabolism; Anti-Bacterial Agents - metabolism; Bacteria - metabolism; Bacterial Proteins - metabolism; Polyketide Synthases - metabolism; Polyketides - metabolism; Pyridones - metabolism; antibiotic; kirromycin; click chemistry; polyketide synthase; trans-acyltransferase; engineering

During polyketide biosynthesis, acyltransferases (ATs) are the essential gatekeepers which provide the assembly lines with precursors and thus contribute greatly to structural diversity. Previously, we demonstrated that the discrete AT KirCII from the kirromycin antibiotic pathway accesses nonmalona...