Polyketide Bioderivatization Using the Promiscuous Acyltransferase KirCII

During polyketide biosynthesis, acyltransferases (ATs) are the essential gatekeepers which provide the assembly lines with precursors and thus contribute greatly to structural diversity. Previously, we demonstrated that the discrete AT KirCII from the kirromycin antibiotic pathway accesses nonmalona...

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Published inACS synthetic biology Vol. 6; no. 3; pp. 421 - 427
Main Authors Musiol-Kroll, Ewa M, Zubeil, Florian, Schafhauser, Thomas, Härtner, Thomas, Kulik, Andreas, McArthur, John, Koryakina, Irina, Wohlleben, Wolfgang, Grond, Stephanie, Williams, Gavin J, Lee, Sang Yup, Weber, Tilmann
Format Journal Article
LanguageEnglish
Published United States American Chemical Society 17.03.2017
Subjects
Acyltransferases - metabolism
Anti-Bacterial Agents - metabolism
Bacteria - metabolism
Bacterial Proteins - metabolism
Polyketide Synthases - metabolism
Polyketides - metabolism
Pyridones - metabolism
antibiotic
kirromycin
click chemistry
polyketide synthase
trans-acyltransferase
engineering
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Summary:During polyketide biosynthesis, acyltransferases (ATs) are the essential gatekeepers which provide the assembly lines with precursors and thus contribute greatly to structural diversity. Previously, we demonstrated that the discrete AT KirCII from the kirromycin antibiotic pathway accesses nonmalonate extender units. Here, we exploit the promiscuity of KirCII to generate new kirromycins with allyl- and propargyl-side chains in vivo, the latter were utilized as educts for further modification by “click” chemistry.
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ISSN:2161-5063
2161-5063
DOI:10.1021/acssynbio.6b00341