Bovine β-Casein (1−28), a Casein Phosphopeptide, Enhances Proliferation and IL-6 Expression of Mouse CD19+ Cells via Toll-like Receptor 4

• Published in: Journal of agricultural and food chemistry Vol. 54; no. 21; pp. 8013 - 8017
• Main Authors: Tobita, Keisuke, Kawahara, Takeshi, Otani, Hajime
• Format: Journal Article
• Language: English
• Published: Washington, DC American Chemical Society 18.10.2006
• Subjects: Animals; Antibodies - pharmacology; Antigens, CD19 - analysis; Biological and medical sciences; Caseins - pharmacology; Cattle; Cell Division - drug effects; Food industries; Fundamental and applied biological sciences. Psychology; Gene Expression - drug effects; Interleukin-6 - genetics; Lymphocytes - drug effects; Lymphocytes - immunology; Male; Mice; Mice, Inbred C3H; Milk and cheese industries. Ice creams; Peptide Fragments - pharmacology; Reverse Transcriptase Polymerase Chain Reaction; Toll-Like Receptor 4 - immunology; Toll-Like Receptor 4 - physiology; Bovine; Casein phosphopeptide; Rodentia; Phosphopeptide; Dairy product; mitogenic activity; Proliferation; IL-6; CD19+ cells; Milk protein; Vertebrata; Mammalia; Casein; Mouse; Artiodactyla; Cell; toll-like receptor 4; Ungulata; Milk
• ISSN: 0021-8561; 1520-5118
• DOI: 10.1021/jf0610864

This study was conducted to elucidate the target cells and receptors which participate in the mitogenic and interleukin (IL)-6-enhancing effect of bovine β-casein (1−28), a casein phosphopeptide. When the spleen lymphocyte subset (CD4+, CD8+, and CD19+ cells) from C3H/HeN mice was cultured with the β-casein (1−28), it exerted a dose-dependent mitogenic effect on CD19+ cells. The effect of β-casein (1−28) was not apparent in the case of CD19+ cells from C3H/HeJ mouse. In addition, the effect was significantly inhibited by treating the C3H/HeN mouse-derived CD19+ cells with neutralizing antibody for toll-like receptor 4 (TLR4). Reverse transcription-polymerase chain reaction analysis showed that the β-casein (1−28) exerted an IL-6-enhancing effect on the CD19+ cells. The effect was also abrogated in either C3H/HeJ mouse-derived CD19+ cell culture or the anti-TLR4 antibody-added culture. These results suggest that the β-casein (1−28) stimulates both proliferation and IL-6 expression of CD19+ cells via TLR4. Keywords: Casein phosphopeptide; milk; mitogenic activity; CD19+ cells; IL-6; toll-like receptor 4